Human Arylsulfatase B/ARSB Antibody

Catalog #: MAB4415
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4415
MAB4415-SP

Detection of Human Arylsulfatase B/ARSB by Western Blot.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Arylsulfatase B/ARSB Antibody Summary

Species Reactivity

Human

Specificity

Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse ARSG is observed.

Source

Monoclonal Mouse IgG₁ Clone # 708401

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
Ser37-Met533
Accession # P15848

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Arylsulfatase B/ARSB by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Arylsulfatase B/ARSB at approximately 47 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Arylsulfatase B/ARSB in HepG2 Human Cell Line. Arylsulfatase B/ARSB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase B/ARSB

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).

References

Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.

Entrez Gene IDs

411 (Human); 11881 (Mouse); 25227 (Rat)

Alternate Names

ARSB; Arylsulfatase B; ASB; EC 3.1.6.12; G4S; MPS6; N-acetylgalactosamine-4-sulfatase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Arylsulfatase B/ARSB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Correction: Nuclear Legumain Activity in Colorectal Cancer
Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
PLoS ONE
Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions
Authors: NN Lunde, MH Haugen, KB Bodin Lars, I Damgaard, SJ Pettersen, R Kasem, W Rut, M Drag, M Poreba, HT Johansen, R Solberg
Biochimie, 2017-05-17;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
Authors: Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H
PLoS ONE, 2014-01-08;9(1):e85721.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Nuclear Legumain Activity in Colorectal Cancer
Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
PLoS ONE