Human Arylsulfatase A/ARSA Biotinylated Antibody

Catalog #: BAF2485
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2485

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Arylsulfatase A/ARSA Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Arylsulfatase A/ARSA in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ARSA is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ARSA
Arg19-Ala507
Accession # AAH14210

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase A/ARSA

As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.

References

Lukatela, G. et al. (1998) Biochemistry 37:3654.
Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

Entrez Gene IDs

410 (Human); 11883 (Mouse)

Alternate Names

ARSA; Arylsulfatase A; ASA; Cerebroside 3-sulfatase; Cerebroside-sulfatase; EC 3.1.6; EC 3.1.6.8; MLD

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Arylsulfatase A/ARSA Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Sulfatide levels correlate with severity of neuropathy in metachromatic leukodystrophy
Authors: Christine í Dali, Norman W. Barton, Mohamed H. Farah, Mihai Moldovan, Jan‐Eric Månsson, Nitin Nair et al.
Annals of Clinical and Translational Neurology