Human Arylsulfatase A/ARSA Antibody

Catalog #: MAB2485
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2485
MAB2485-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Arylsulfatase A/ARSA Antibody Summary

Species Reactivity

Human

Specificity

Detects human Arylsulfatase A/ARSA in direct ELISAs and Western blots. In direct ELISAs and Western blots, 20‑50% cross‑reactivity with recombinant mouse ARSA is observed.

Source

Monoclonal Mouse IgG_2B Clone # 324802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Arylsulfatase A/ARSA
Arg19-Ala507
Accession # AAH14210

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase A/ARSA

As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Human ARSA has a predicted molecular mass of approximately 53 kDa and an apparent molecular mass of approximately 63 kDa in SDS-PAGE. The amino acid sequence of human ARSA shares 92%, 89%, 87%, and 86% identity with porcine, canine, mouse, and rat ARSA.

References

Lukatela, G. et al. (1998) Biochemistry 37:3654.
Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

Entrez Gene IDs

410 (Human); 11883 (Mouse)

Alternate Names

ARSA; Arylsulfatase A; ASA; Cerebroside 3-sulfatase; Cerebroside-sulfatase; EC 3.1.6; EC 3.1.6.8; MLD

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Arylsulfatase A/ARSA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A conserved long-distance telomeric silencing mechanism suppresses mTOR signaling in aging human fibroblasts
Authors: K Jäger, J Mensch, ME Grimmig, B Neuner, K Gorzelniak, S Türkmen, I Demuth, A Hartmann, C Hartmann, F Wittig, A Sporbert, A Hermann, G Fuellen, S Möller, M Walter
Science Advances, 2022-08-17;8(33):eabk2814.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot