Human Apolipoprotein B100/ApoB100 Biotinylated Antibody

Catalog #: BAF3260 Datasheet / COA / SDS

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BAF3260

All Apolipoprotein B100/ApoB100 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein B100/ApoB100 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apoliprotein B100 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Apoliprotein B100
Ile3374-Val3600
Accession # AAP72970

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Apolipoprotein B100/ApoB100

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B100/ApoB100

ApoB100 is a major serum apoliprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4; Apolipoprotein B100