Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

Catalog # Availability Size / Price Qty
MAB38292-100
MAB38292-SP
Detection of Human Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot.
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Human Angiopoietin-like Protein 3/ANGPTL3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin‑like Protein 3/ANGPTL3 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 388610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin‑like Protein 3/ANGPTL3
Ile19-Glu460
Accession # Q9Y5C1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Angiopoietin-like Protein 3/ANGPTL3 antibody by Western Blot. View Larger

Detection of Human Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line (negative control), HepG2 human hepatocellular carcinoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38292) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70 kDa, 50 kDa, and 32 kDa species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% amino acid sequence identity with mouse ANGPTL3.

Entrez Gene IDs
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

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