Human Adiponectin/Acrp30 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1065
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Human Adiponectin/Acrp30 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adiponectin in ELISAs. In ELISAs, this antibody shows no cross-reactivity with recombinant mouse Adiponectin. This antibody detects an epitope in the globular domain (amino acids 104 - 244).
Source
Monoclonal Mouse IgG1 Clone # 166128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Adiponectin
Glu19-Asn244
Accession # Q15848
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human Adiponectin/Acrp30 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651R)

Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651)

Standard: Recombinant Human Adiponectin/Acrp30 Protein, CF (Catalog # 1065-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as adipocyte complement-related protein of 30 kDa (Acrp30), is secreted from adipocytes and has been implicated in energy homeostasis and obesity.

Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

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Citations for Human Adiponectin/Acrp30 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The Impact of an 8-Week Supplementation with Fermented and Non-Fermented Aronia Berry Pulp on Cardiovascular Risk Factors in Individuals with Type 2 Diabetes
    Authors: Christiansen, CB;Jeppesen, PB;Hermansen, K;Gregersen, S;
    Nutrients
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  2. Nutritional Factors Modulating Alu Methylation in an Italian Sample from The Mark-Age Study Including Offspring of Healthy Nonagenarians
    Authors: R Giacconi, M Malavolta, A Bürkle, M Moreno-Vil, C Franceschi, M Capri, PE Slagboom, EHJM Jansen, MET Dollé, T Grune, D Weber, A Hervonen, W Stuetz, N Breusing, F Ciccarone, M Zampieri, V Aversano, P Caiafa, L Formentini, F Piacenza, E Pierpaoli, A Basso, M Provincial, M Cardelli
    Nutrients, 2019-12-06;11(12):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  3. Dopamine Receptors in Human Adipocytes: Expression and Functions
    Authors: Dana C. Borcherding, Eric R. Hugo, Gila Idelman, Anuradha De Silva, Nathan W. Richtand, Jean Loftus et al.
    PLoS ONE
  4. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  5. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  6. Adiponectin levels measured in dried blood spot samples from neonates born small and appropriate for gestational age.
    Authors: Klamer A, Skogstrand K, Hougaard DM, Norgaard-Petersen B, Juul A, Greisen G
    Eur. J. Endocrinol., 2007-08-01;157(2):189-94.
    Species: Human
    Sample Types: Dried Blood Spot Sample (DBSS)
    Applications: Luminex Development
  7. Rosiglitazone treatment increases plasma levels of adiponectin and decreases levels of resistin in overweight women with PCOS: a randomized placebo-controlled study.
    Authors: Majuri A, Santaniemi M, Rautio K, Kunnari A, Vartiainen J, Ruokonen A, Kesaniemi YA, Tapanainen JS, Ukkola O, Morin-Papunen L
    Eur. J. Endocrinol., 2007-02-01;156(2):263-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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