Human ADAM32 Antibody

Catalog #: AF5380 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5380
AF5380-SP

Detection of Human ADAM32 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM32 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM32 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ADAM9, rhADAM22, and rhADAM23 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Ser17-Thr476
Accession # Q8TC27

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAM32, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ADAM32 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ADAM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAM32 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM32

ADAM32 (a disintegrin and metalloprotease domain 32) is a 90‑100 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin-binding disintegrin region (aa 391‑479), a Cys-rich segment (aa 480 - 502) and an EGF-like domain (aa 622‑654). In the testis, mature ADAM32 is approximately 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Long Name

A Disintegrin and Metalloproteinase Domain 32

Entrez Gene IDs

203102 (Human); 353188 (Mouse); 361170 (Rat)

Alternate Names

a disintegrin and metalloprotease domain 32; a disintegrin and metalloproteinase domain 32; ADAM 32; ADAM metallopeptidase domain 32; ADAM32; disintegrin and metalloproteinase domain-containing protein 32; FLJ26299; FLJ29004; metalloproteinase 12-like protein