Human Activin C beta C subunit Antibody

Catalog #: MAB1629 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1629
MAB1629-SP

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Human Activin C beta C subunit Antibody Summary

Species Reactivity

Human

Specificity

Detects human Activin C beta C subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Activin A, B, AB, rhInhibin A, B, or recombinant mouse Activin C is observed.

Source

Monoclonal Mouse IgG₁ Clone # 203522

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Activin C beta C subunit
Gly237-Ser352
Accession # P55103

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Activin C beta C subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1629).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Activin C ELISA Standard Curve. Recombinant Human Activin C protein was serially diluted 2-fold and captured by Mouse Anti-Human Activin C beta C subunit Monoclonal Antibody (Catalog # MAB1629) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Activin C beta C subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1629) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin C

Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of Activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta _c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported (1).

References