Human Activin C Antibody

Catalog # Availability Size / Price Qty
MAB11501-100
MAB11501-SP
Detection of Activin C in Human Liver.
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Human Activin C Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin C in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1077920
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Activin C
Gly237-Ser352
Accession # P55103
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human liver
Immunocytochemistry
3-25 µg/mL
 fixed HepG2 human hepatocellular carcinoma cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Activin C in Human Liver. Activin C was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of liver hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry View Larger

Detection of Activin C in HepG2 cells (Positive) and THP-1 cells (Negative). Activin C was detected in fixed HepG2 human hepatocellular carcinoma cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin C

Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of Activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported (1).

References
  1. Mellor, S. et al. (2003) Endocrinology 144:4410.
Entrez Gene IDs
3626 (Human); 16325 (Mouse)
Alternate Names
inhibin beta C chain; Activin C; IHBC; INHBC; inhibin, beta C

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