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Canine HGFR/c-MET Antibody

Catalog #: AF4140
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4140
AF4140-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine HGFR/c-MET Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine HGF R/c‑MET in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human HGF R and remombinant mouse HGF R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine HGF R/c‑MET
Glu25-Leu935
Accession # Q75ZY9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Canine HGF R/c-MET (Catalog # 4140-ME)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Canine HGF to immobilized Recombinant Canine HGF R/c-MET (Catalog # 4140-ME) coated at 2 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
 

Canine HGF R/c-MET Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Canine HGFR/c-MET Biotinylated Antibody (Catalog # BAF4140)

Standard: Recombinant Canine HGFR/c-MET Protein, CF (Catalog # 4140-ME)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes posttranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS region, and four Ig-like E-set domains, while the cytoplasmic region includes a tyrosine kinase domain (3). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 4). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (5, 6). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (7). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin alpha 6/ beta 4, plexins B1, B2, and B3, and MSP R/Ron (8‑15). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (8‑15). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (8, 12, 13). HGF released from neighboring mesenchymal cells stimulates HGF R on undifferentiated epithelium and induces epithelial cell scattering and branching tubulogenesis (16). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, canine HGF R shares 85%‑88% amino acid sequence identity with human, mouse and rat HGF R.

 

References
  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  5. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  6. Ponzetto, C. et al. (1994) Cell 77:261.
  7. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  8. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  9. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  10. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  11. Wang, X. et al. (2002) Mol. Cell 9:411.
  12. Trusolino, L. et al. (2001) Cell 107:643.
  13. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  14. Conrotto, P. et al. (2004) Oncogene 23:5131.
  15. Follenzi, A. et al. (2000) Oncogene 19:3041.
  16. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Entrez Gene IDs
4233 (Human); 17295 (Mouse)
Alternate Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Citations for Canine HGFR/c-MET Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis
    Authors: JW Clancy, CS Sheehan, CJ Tricarico, C D'Souza-Sc
    J. Biol. Chem., 2018-06-14;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: ICC
  2. Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
    Authors: Abella JV, Parachoniak CA, Sangwan V, Park M
    J. Biol. Chem., 2010-06-07;285(32):24956-67.
    Species: Canine
    Sample Types: Whole Cells
    Applications: ICC

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