Turning Flow Cytometry Upside Down and Inside Out: Cellular Function Revealed

Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies. Multi-parameter, fluidic-based flow cytometry has been applied to multiple fields of study ranging from basic to clinical immunology, cancer biology, neuroscience, stem cell research, and drug discovery. In order to maximize the quality of results obtained using this technology, researchers should be familiar with the basics of flow cytometry when setting up an experiment. In this series of seminars, we will discuss the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment.

Webinar 2 - Turning Flow Cytometry Upside Down and Inside Out: Cellular Function Revealed

In this webinar, you will learn how to apply basic concepts of flow cytometry to topics such as cell proliferation, dead cell exclusion, and intracellular staining techniques for transcription factors and phospho-proteins. You will learn the importance of:

Titrating cell proliferation dyes and flow antibodies for optimal results
How dead cell exclusion and staining temperatures of surface markers can influence interpretation of your data
Different fixation and permeabilization buffers and their use in detecting transcription factors and secreted cytokines vs. phospho-proteins

An in-depth discussion of these techniques will be illustrated in a multi-parameter flow experiment examining human memory T cell subsets.