Human ICAM-1/CD54 Antibody

Name: Human ICAM-1/CD54 Antibody
Brand: R&D Systems
SKU: MAB720
Rating: 4.7 (3 reviews)

Catalog #: MAB720
5 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB720-SP
MAB720-100
MAB720-500

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All ICAM-1/CD54 Antibodies

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-1/CD54 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.

Source

Monoclonal Mouse IgG₁ Clone # 14C11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ICAM‑1/CD54 (Catalog # ADP4)
under non-reducing conditions only

Immunocytochemistry

5-25 µg/mL

See below

Human ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF720)

Standard: Recombinant Human ICAM-1/CD54 Protein, CF (Catalog # ADP4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

ICAM‑1/CD54 in A431 Human Cell Line. ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be upregulated upon cell activation.

Long Name

Intercellular Adhesion Molecule 1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Alternate Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation
Authors: Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al.
PLoS Pathogens
High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria.
Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel
PLoS ONE, 2013-12-27;8(12):e84181.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development
Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium
Authors: I Gomez, B Ward, C Souilhol, C Recarti, M Ariaans, J Johnston, A Burnett, M Mahmoud, LA Luong, L West, M Long, S Parry, R Woods, C Hulston, B Benedikter, C Niespolo, R Bazaz, S Francis, E Kiss-Toth, M van Zandvo, A Schober, P Hellewell, PC Evans, V Ridger
Nat Commun, 2020-01-10;11(1):214.