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Human CCL28 Antibody

Catalog #: MAB7171
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7171
MAB7171-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL28 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL28 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL27 or recombinant mouse CCL28 is observed.
Source
Monoclonal Mouse IgG1 Clone # 62714
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL28 (Catalog # 717-VC)

Human CCL28 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL28 Biotinylated Antibody (Catalog # BAF717)

Standard: Recombinant Human CCL28 Protein (Catalog # 717-VC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine superfamily. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 share 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA is most highly expressed by epithelial cells in normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is the CCR10 (formerly GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References
  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Entrez Gene IDs
56477 (Human); 56838 (Mouse)
Alternate Names
CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

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Citations for Human CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
    Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
    Mucosal Immunol, 2020-04-28;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma
    Authors: Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al.
    Laboratory Investigation
  3. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
    Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
    Clin Cancer Res, 2015-02-23;21(12):2840-50.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization

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