Human CD25/IL-2R alpha Antibody Summary
Glu22-Cys213
Accession # P01589
Applications
Human CD25/IL-2 R alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
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CD25/IL‑2 R alpha in Human PBMCs. CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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CD25/IL‑2 R alpha in Human Lymph Node. CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
Product Datasheets
Citations for Human CD25/IL-2R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
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UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling
Authors: Torrino S, Tiroille V, Dolfi B Et al.
eLife
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Nicotinamide mononucleotide impacts HIV-1 infection by modulating immune activation in T lymphocytes and humanized mice
Authors: Mo, Y;Yue, M;Yim, LY;Zhou, R;Yu, C;Peng, Q;Zhou, Y;Luk, TY;Lui, GC;Huang, H;Lim, CYH;Wang, H;Liu, L;Sun, H;Wang, J;Song, Y;Chen, Z;
EBioMedicine
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
Sustained in vivo signaling by long-lived IL-2 induces prolonged increases of regulatory T cells
Authors: Charles J.M. Bell, Yongliang Sun, Urszula M. Nowak, Jan Clark, Sarah Howlett, Marcin L. Pekalski et al.
Journal of Autoimmunity
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Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain.
Authors: Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M
Fertil. Steril., 2007-10-24;89(6):1618-24.
Species: Human
Sample Types: Peritoneal Fluid
Applications: ELISA Development -
Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
Biochem. J., 2004-06-01;380(0):425-33.
Species: Human
Sample Types:
Applications: Western Blot
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