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Human BMP-6 Antibody

Catalog #: MAB507
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB507-SP
MAB507-100
MAB507-500
Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-6 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP‑2, rhBMP‑4, rhBMP‑5, or rhBMP‑7 is observed.
Source
Monoclonal Mouse IgG2B Clone # 74219
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP‑6
Arg296-His428
Accession # Q4VBA3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BMP‑6 (Catalog # 507-BP) under non-reducing conditions only

Human BMP-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human BMP‑6 Biotinylated Antibody (Catalog # BAF507)

Standard: Recombinant Human BMP-6 Protein (Catalog # 507-BP)

Neutralization
Measured by its ability to neutralize BMP‑6-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 300 ng/mL Recombinant Human BMP‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. Recombinant Human BMP-6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-6 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-6 Monoclonal Antibody (Catalog # MAB507). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7, and -8 are in the same subgroup.

References
  1. Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
  2. Reddi, A.H. (1998) Nature Biotechnology 16:247.
Long Name
Bone Morphogenetic Protein 6
Entrez Gene IDs
654 (Human); 12161 (Mouse); 25644 (Rat)
Alternate Names
BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1

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Citations for Human BMP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Live-cell invasive phenotyping uncovers the ALK2/BMP6 iron homeostasis pathway as a therapeutic vulnerability in LKB1-mutant lung cancer
    Authors: Koo J, Seong CS, Parker RE et al.
    bioRxiv
  2. Smad1/5 is required for erythropoietin-mediated suppression of hepcidin in mice
    Authors: CY Wang, AB Core, S Canali, KB Zumbrennen, S Ozer, L Umans, A Zwijsen, JL Babitt
    Blood, 2017-04-24;0(0):.
  3. Live-Cell Invasive Phenotyping Uncovers ALK2 as a Therapeutic Target in LKB1-Mutant Lung Cancer
    Authors: Koo, J;Seong, CS;Parker, RE;Herrera, A;Dwivedi, B;Arthur, RA;Dinasarapu, AR;Johnston, HR;Claussen, H;Tucker-Burden, C;Ramalingam, SS;Fu, H;Zhou, W;Marcus, AI;Gilbert-Ross, M;
    Cancer research
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Live-cell invasive phenotyping uncovers the ALK2/BMP6 iron homeostasis pathway as a therapeutic vulnerability in LKB1-mutant lung cancer
    Authors: Koo J, Seong CS, Parker RE et al.
    bioRxiv
  5. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
    Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
    Oncogene, 2018-04-26;37(29):4013-4032.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. TNF? drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling
    Authors: LA Hurst, BJ Dunmore, L Long, A Crosby, R Al-Lamki, J Deighton, M Southwood, X Yang, MZ Nikolic, B Herrera, GJ Inman, JR Bradley, AA Rana, PD Upton, NW Morrell
    Nat Commun, 2017-01-13;8(0):14079.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Regulation of TMPRSS6 by BMP6 and iron in human cells and mice.
    Authors: Meynard D, Vaja V, Sun CC, Corradini E, Chen S, Lopez-Otin C, Grgurevic L, Hong CC, Stirnberg M, Gutschow M, Vukicevic S, Babitt JL, Lin HY
    Blood, 2011-05-26;118(3):747-56.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
    Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
    Cancer Res., 2005-09-15;65(18):8274-85.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot

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