Human CCL21/6Ckine Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF366
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human CCL21/6Ckine Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL21/6Ckine in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) TARC, rhMIP‑1δ, rhEotaxin‑2, rhTECK, and recombinant mouse 6Ckine is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL21/6Ckine (R&D Systems, Catalog # 366-6C)
Ser24-Pro134
Accession # Q6ICR7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL21/6Ckine (Catalog # 366-6C)

Human CCL21/6Ckine Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL21/6Ckine Antibody (Catalog # MAB3661)

Standard: Recombinant Human CCL21/6Ckine Protein (Catalog # 366-6C)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus-2, shows 21 - 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for MIP‑3 beta /ELC, another novel CC chemokine.

Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines so far tested.

References
  1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
Entrez Gene IDs
6366 (Human); 18829 (Mouse); 298006 (Rat)
Alternate Names
6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4

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Citations for Human CCL21/6Ckine Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Analysis of microRNA expression in the thymus of Myasthenia Gravis patients opens new research avenues
    Authors: Mélanie A. Cron, Solène Maillard, Fabien Delisle, Nolwenn Samson, Frédérique Truffault, Maria Foti et al.
    Autoimmunity Reviews
  2. CXCL12 promotes CCR7 ligand–mediated breast cancer cell invasion and migration toward lymphatic vessels
    Authors: Haruko Hayasaka, Junichi Yoshida, Yasutaka Kuroda, Akihiro Nishiguchi, Michiya Matsusaki, Kei Kishimoto et al.
    Cancer Science
  3. CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
    Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
    Ann. Neurol., 2009-10-01;66(4):521-31.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  4. Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
    Authors: Page G, Miossec P
    J. Pathol., 2004-09-01;204(1):28-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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