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Human IL-29/IFN-lambda 1 Biotinylated Antibody

Catalog #: BAF1598
4 Citations 2 Reviews Datasheet / COA / SDS
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BAF1598
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-29/IFN-lambda 1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑29/IFN‑ lambda 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 100% cross-reactivity with recombinant human (rh) IL-28B is observed, 35% cross-reactivity with rhIL-28A is observed and less than 0.2% cross‑reactivity with rhIFN‑ alpha A, rhIFN-alpha B2, rhIFN‑ alpha G, rhIFN alpha I, and rhIFN-beta 1A is observed. In Western blots, approximately 25% cross-reactivity with rhIL-28A and rhIL‑28B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑29/IFN‑ lambda 1
Gly20-Thr200
Accession # Q8IU54
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑29/IFN-lambda 1 (Catalog # 1598-IL)

IL-29/IFN-lambda 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑29/IFN‑ lambda 1 Antibody (Catalog # MAB15981)

Standard: Recombinant Human IL-29/IFN-lambda 1 Protein (Catalog # 1598-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-29/IFN-lambda 1

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL‑29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor  beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.

References
  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Long Name
Interleukin 29
Entrez Gene IDs
282618 (Human)
Alternate Names
cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL29; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21

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Citations for Human IL-29/IFN-lambda 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. High levels of circulating interferons type I, type II and type III associate with distinct clinical features of active systemic lupus erythematosus
    Authors: V Oke, I Gunnarsson, J Dorschner, S Eketjäll, A Zickert, TB Niewold, E Svenungsso
    Arthritis Res. Ther., 2019-04-29;21(1):107.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  2. IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE)
    Authors: V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso
    Arthritis Res. Ther., 2017-06-15;19(1):139.
    Species: Human
    Sample Types: Serum
  3. Interferon (IFN)-? is a potential mediator in lupus nephritis
    Authors: Iva Gunnarsson
    Lupus Sci Med, 2016-11-22;3(1):e000170.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  4. Human TLR-7-, -8-, and -9-mediated induction of IFN-alpha/beta and -lambda Is IRAK-4 dependent and redundant for protective immunity to viruses.
    Authors: Yang K, Puel A, Zhang S, Eidenschenk C, Ku CL, Casrouge A, Picard C, von Bernuth H, Senechal B, Plancoulaine S, Al-Hajjar S, Al-Ghonaium A, Marodi L, Davidson D, Speert D, Roifman C, Garty BZ, Ozinsky A, Barrat FJ, Coffman RL, Miller RL, Li X, Lebon P, Rodriguez-Gallego C, Chapel H, Geissmann F, Jouanguy E, Casanova JL
    Immunity, 2005-11-01;23(5):465-78.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Reviews for Human IL-29/IFN-lambda 1 Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human IL-29/IFN-lambda 1 Biotinylated Antibody
By Anonymous on 09/12/2022
Application: ELISA Sample Tested: Serum Species: Human
ELISA Human IL-29/IFN-lambda 1 Biotinylated Antibody BAF1598
Human IL-29/IFN-lambda 1 Biotinylated Antibody
By Anonymous on 06/01/2018
Application: ELISA Sample Tested: Serum Species: Human

This antibody served as the detection in a sandwich ELISA with MAB15981 as the capture.

ELISA Human IL-29/IFN-lambda 1 Biotinylated Antibody BAF1598