Mouse IL-17E/IL-25 Biotinylated Antibody

Catalog #: BAF1399
4 Citations Datasheet / COA / SDS

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BAF1399

All IL-17E/IL-25 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17E/IL-25 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑17E/IL-25 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity recombinant human IL‑17E, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17F is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL‑17E/IL-25 (R&D Systems, Catalog # 1399-IL)
Val17-Ala169
Accession # NP_542767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL)

Mouse IL-17E/IL-25 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IL‑17E/IL‑25 Antibody (Catalog # MAB13991)

Standard: Recombinant Mouse IL-17E Protein (Catalog # 1399-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Pan, G. et al. (2001) J. Immunol. 167:6569.

Long Name

Interleukin 17E

Entrez Gene IDs

64806 (Human); 140806 (Mouse); 501996 (Rat)

Alternate Names

IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-17E/IL-25 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation
Authors: S Li, X Ding, H Zhang, Y Ding, Q Tan
International immunopharmacology, 2022-02-08;106(0):108605.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway
Authors: HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim
Arthritis Research & Therapy, 2020-09-23;22(1):222.
Species: Human
Sample Types: Synovial Fluid
IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice
Authors: T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae
Sci Rep, 2019-11-19;9(1):17067.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
IL-25 enhances allergic airway inflammation by amplifying a TH2 cell-dependent pathway in mice.
Authors: Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokuhisa T, Iwamoto I, Nakajima H
J. Allergy Clin. Immunol., 2006-06-21;118(3):606-14.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development