Mouse Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

Catalog #: BAF136 Datasheet / COA / SDS

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BAF136

Angiopoietin‑like Protein 3/ANGPTL3 in mouse liver.

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All Angiopoietin-like Protein 3/ANGPTL3 Antibodies

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Mouse Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Angiopoietin-like 3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) Angiopoietin-like 3 (4954-AN and 3829-AN), recombinant mouse (rm) Angiopoietin-3, and rmAngiopoietin-like 4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Angiopoietin-like 3
Ser17-Thr455
Accession # Q9R182

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Angiopoietin-like 3 (Catalog # 136-AN)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse liver

Mouse Angiopoietin-like Protein 3/ANGPTL3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Angiopoietin-like 3 Antibody (Catalog # MAB13621)

Standard: Recombinant Mouse Angiopoietin-like 3 Protein, CF (Catalog # 136-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Angiopoietin‑like Protein 3/ANGPTL3 in mouse liver. Angiopoietin‑like Protein 3/ANGPTL3 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1‑3). Mature mouse ANGPTL3 contains an N‑terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled-coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin‑binding motif that is N‑terminal to the coiled‑coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6‑8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is up‑regulated by LXR agonists and down‑regulated by insulin, leptin, and TR beta agonists (10‑12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22%‑30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

References

Li, C. (2006) Curr. Opin. Lipidol. 17:152.
Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
Conklin, D. et al. (1999) Genomics 62:477.
Ge, H. et al. (2005) J. Lipid Res. 46:1484.
Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
Koishi, R. et al. (2002) Nat. Genet. 30:151.
Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
Koster, A. et al. (2005) Endocrinology 146:4943.
Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Alternate Names

AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2