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Mouse CD25/IL-2R alpha Antibody

Catalog #: AF2438
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2438
AF2438-SP
Cell Proliferation Induced by IL‑2 and Neutralization by Mouse CD25IL‑2 R alpha Antibody.
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Citations (3)
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Mouse CD25/IL-2R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
Glu22-Lys236
Accession # Q544I2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD25/IL‑2 R alpha (Catalog # 2438-RM)
Immunohistochemistry
5-15 µg/mL
See below

Mouse CD25/IL-2 R alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CD25/IL-2R alpha Biotinylated Antibody (Catalog # BAF2438)

Standard: Recombinant Mouse CD25/IL-2R alpha Protein, CF (Catalog # 2438-RM)

Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 2 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Mouse CD25IL‑2 R alpha Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse CD25IL‑2 R alpha Antibody. Recombinant Mouse IL-2 (402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND50 is typically 5-20 µg/mL.

Immunohistochemistry CD25/IL-2 Ra antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

CD25/IL‑2 R alpha in Mouse Spleen. CD25/IL-2 Ra was detected in immersion fixed frozen sections of mouse spleen using 5 µg/mL Goat Anti-Mouse CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-14).

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
  4. Miller, J. et al. (1985) J. Immunol. 134:4212.
  5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
  6. Hatakeyama, M. et al. (1989) Science 244:551.
  7. Takeshita, T. et al. (1992) Science 257:379.
  8. Wang, X. et al. (2005) Science 310:1159.
  9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
  12. Willerford, D.M. et al. (1995) Immunity 3:521.
  13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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Citations for Mouse CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Aspergillus fumigatus Influences Gasdermin-D-Dependent Pyroptosis of the Lung via Regulating Toll-Like Receptor 2-Mediated Regulatory T Cell Differentiation
    Authors: Wei Yan, Yi-si Zhao, Ke Xie, Yu Xing, Fang Xu
    Journal of Immunology Research
  2. Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury
    Authors: K Xie, YS Chai, SH Lin, F Xu, CJ Wang
    Journal of Immunology Research, 2021-01-18;2021(0):8883962.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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