TGF-beta 2 Antibody

Detection of Human TGF‑ beta 2 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TGF‑ beta 2 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF‑ beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody. Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND₅₀ is typically 0.25-1.25 µg/mL.

Detection of Human TGF-beta 2 Antibody by Western Blot MiR-30b induces expression of TGF beta 2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta 1 and TGF beta 2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta -actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta 2 protein levels by western blot. beta -actin was used as endogenous control. (C) ELISAs for TGF beta 1 and TGF beta 2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TGF-beta 2 Antibody by Western Blot MiR-30b induces expression of TGF beta 2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta 1 and TGF beta 2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta -actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta 2 protein levels by western blot. beta -actin was used as endogenous control. (C) ELISAs for TGF beta 1 and TGF beta 2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.