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TGF-beta 1/1.2 Antibody

Catalog #: AF-101-NA
44 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-101-NA
AF-101-SP
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody.
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Citations (44)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 1/1.2 Antibody Summary

Specificity
Detects TGF-beta 1/1.2 in direct ELISAs and Western blots.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1
Ala279-Ser390
Accession # P01137
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
Neutralization
Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically ≤500 ng/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody. View Larger

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody. Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF-beta 1/1.2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-101-NA). The ND50 is typically ≤100 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1/1.2

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Long Name
Transforming Growth Factor beta 1 and 1.2
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat)
Alternate Names
TGFb1.2; TGF-beta 1/1.2

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Citations for TGF-beta 1/1.2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts
    Authors: P Uruski, J Miku?a-Pie, M Paku?a, S Budkiewicz, M Drzewiecki, AN Gaiday, M Wierzowiec, E Naumowicz, R Moszy?ski, A Tykarski, K Ksi??ek
    International Journal of Molecular Sciences, 2021-04-19;22(8):.
  2. Age Related Changes in Muscle Mass and Force Generation in the Triple Transgenic (3xTgAD) Mouse Model of Alzheimer’s Disease
    Authors: Hongyang Xu, Shylesh Bhaskaran, Katarzyna M. Piekarz, Rojina Ranjit, Jan Bian, Parker Kneis et al.
    Frontiers in Aging Neuroscience
  3. Myocardial Hypertrophy and Compensatory Increase in Systolic Function in a Mouse Model of Oxidative Stress
    Authors: Rohan Varshney, Rojina Ranjit, Ying Ann Chiao, Michael Kinter, Bumsoo Ahn
    International Journal of Molecular Sciences
  4. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
    Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
    Nature Neuroscience, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Aggressive Mammary Cancers Lacking Lymphocytic Infiltration Arise in Irradiated Mice and Can Be Prevented by Dietary Intervention
    Authors: Coral Omene, Lin Ma, Jade Moore, Haoxu Ouyang, Irineu Illa-Bochaca, William Chou et al.
    Cancer Immunology Research
  6. Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells
    Authors: M Paku?a, E Ma?y, P Uruski, A Witucka, M Bogucka, N Jaroszewsk, N Makowska, A Niklas, R Moszy?ski, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2020-01-27;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Neutralization of transforming growth factor (TGF)-&beta1 activity reduced fibrosis and enhanced regeneration of glycerol-injured rat muscle
    Authors: MAA Mahdy, K Warita, YZ Hosaka
    J. Vet. Med. Sci., 2019-12-25;82(2):168-171.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Oral Tolerance Induction to Newly Introduced Allergen is Favored by a Transforming Growth Factor-beta-Enriched Formula
    Authors: S Holvoet, M Perrot, N de Groot, G Prioult, T Mikogami, V Verhasselt, S Nutten
    Nutrients, 2019-09-13;11(9):.
    Species: Mink
    Sample Types: Whole Cells
    Applications: Neutralization
  9. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019-05-13;11(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
    Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
    Int J Mol Sci, 2019-01-02;20(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration
    Authors: O Miroshnych, WT Chang, JL Dragoo
    Am J Sports Med, 2016-12-27;45(4):945-953.
    Species: Human
    Sample Types: Platelet Rich Plasma (PRP)
    Applications: Immunoprecipitation
  12. Transforming growth factor-beta inhibits IQ motif containing guanosine triphosphatase activating protein 1 expression in lung fibroblasts via the nuclear factor-kappa B signaling pathway
    Authors: CHUANYUE ZONG, XIANLONG ZHANG, YING XIE, JIAWEN CHENG
    Molecular Medicine Reports
  13. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
    Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J
    Sci Rep, 2015-03-09;5(0):8919.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Prestress in the extracellular matrix sensitizes latent TGF-beta 1 for activation
    Authors: Franco Klingberg, Melissa L. Chow, Anne Koehler, Stellar Boo, Lara Buscemi, Thomas M. Quinn et al.
    Journal of Cell Biology
  15. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  16. IL-21 induces IL-22 production in CD4+ T cells.
    Authors: Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F
    Nat Commun, 2014-05-06;5(0):3753.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Plasma Exosomes as Markers of Therapeutic Response in Patients with Acute Myeloid Leukemia
    Authors: Chang-Sook Hong, Laurent Muller, Theresa L. Whiteside, Michael Boyiadzis
    Frontiers in Immunology
  18. Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
    Authors: Kang I, Yoon D, Braun K, Wight T
    J Biol Chem, 2014-04-09;289(22):15393-404.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy.
    Authors: Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
    PLoS ONE, 2013-12-23;8(12):e83846.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  20. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression.
    Authors: Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A
    Nat Immunol, 2013-10-27;14(12):1256-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  21. Identification of the thiol isomerase-binding peptide, mastoparan, as a novel inhibitor of shear-induced transforming growth factor beta1 (TGF-beta1) activation.
    Authors: Brophy T, Coller B, Ahamed J
    J Biol Chem, 2013-03-05;288(15):10628-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Murine CD4 T Cells Produce a New Form of TGF-beta as Measured by a Newly Developed TGF-beta Bioassay
    Authors: Takatoku Oida, Howard L. Weiner
    PLoS ONE
  23. Adipose tissue derived stem cells differentiate into carcinoma-associated fibroblast-like cells under the influence of tumor derived factors
    Authors: Constantin Jotzu, Eckhard Alt, Gabriel Welte, Jie Li, Bryan T. Hennessy, Eswaran Devarajan et al.
    Cell Oncol (Dordr)
  24. Reciprocal Functional Modulation of the Activation of T Lymphocytes and Fibroblasts Derived from Human Solid Tumors
    Authors: Jennifer L. Barnas, Michelle R. Simpson-Abelson, Stephen P. Brooks, Raymond J. Kelleher, Richard B. Bankert
    The Journal of Immunology
  25. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2010-08-18;185(6):3529-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Transforming growth factor beta (TGFB) signaling is activated during porcine implantation: proposed role for latency-associated peptide interactions with integrins at the conceptus–maternal interface
    Authors: Dana A Massuto, Eric C Kneese, Gregory A Johnson, Robert C Burghardt, R Neil Hooper, Nancy H Ing et al.
    REPRODUCTION
  27. Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues.
    Authors: Vitsky A, Waire J, Pawliuk R, Bond A, Matthews D, Lacasse E, Hawes ML, Nelson C, Richards S, Piepenhagen PA, Garman RD, Andrews L, Thurberg BL, Lonning S, Ledbetter S, Ruzek MC
    Am. J. Pathol., 2009-04-30;174(6):2137-49.
    Species: Mouse
    Sample Types: Saliva
    Applications: Western Blot
  28. Activation of TGF-beta within cultured hepatocytes and in liver injury leads to intracrine signaling with expression of connective tissue growth factor
    Authors: Olav A. Gressner, Birgit Lahme, Monika Siluschek, Katharina Rehbein, Jens Herrmann, Ralf Weiskirchen et al.
    Journal of Cellular and Molecular Medicine
  29. TGF-beta promotes thyroid epithelial cell hyperplasia and fibrosis in IFN-gamma-deficient NOD.H-2h4 mice.
    Authors: Yu S, Sharp GC, Braley-Mullen H
    J. Immunol., 2008-08-01;181(3):2238-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
    Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
    Vaccine, 2008-06-12;26(33):4237-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
    Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
    Am. J. Pathol., 2008-03-18;172(4):1127-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix
    Authors: Pierre-Jean Wipff, Daniel B. Rifkin, Jean-Jacques Meister, Boris Hinz
    The Journal of Cell Biology
  33. Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo.
    Authors: Ruzek MC, Waire JS, Hopkins D, Lacorcia G, Sullivan J, Roberts BL, Richards SM, Nahill SR, Williams JM, Scaria A, Dzuris J, Shankara S, Garman RD
    Blood, 2007-11-19;111(3):1726-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia.
    Authors: Lin J, Patel SR, Wang M, Dressler GR
    Mol. Cell. Biol., 2006-06-01;26(12):4577-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
    Authors: Dasgupta S, Bhattacharya-Chatterjee M, O&amp;apos;Malley BW, Chatterjee SK
    J. Cell. Biochem., 2006-04-01;97(5):1036-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  36. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006-03-30;35(2):252-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006-01-20;31(5):516-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. CD4+CD25+ regulatory T cells inhibit natural killer cell functions in a transforming growth factor-beta-dependent manner
    Authors: François Ghiringhelli, Cédric Ménard, Magali Terme, Caroline Flament, Julien Taieb, Nathalie Chaput et al.
    The Journal of Experimental Medicine
  39. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  40. Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
    Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P
    Neurobiol. Dis., 2005-02-01;18(1):193-205.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  41. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  42. Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
    Authors: Conant SB, Swanborg RH
    J. Immunol., 2004-05-01;172(9):5322-8.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  43. Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
    Authors: Allen SS, Cassone L, Lasco TM
    Infect. Immun., 2004-03-01;72(3):1358-63.
    Species: Guinea Pig
    Sample Types: In Vivo
    Applications: Neutralization
  44. Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
    Authors: Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
    Gene Ther., 2004-01-01;11(2):126-32.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

  1. Does AF-101-NA detect other isoforms of TGF-beta?

    • Cross-reactivity testing results are listed in the Specificity section on the Product Datasheet: less than 15% cross-reactivity with recombinant amphibian TGF-beta 5 and less than 2% with TGF-beta 2 and TGF-beta 3, have been observed, as measured by direct ELISA.

  2. Can pro-IL-1 beta be detected by Catalog #s AF-201-NA and MAB601 ?

    • The amino acid sequence present in mature IL-1 beta (Ala117-Ser269) is also present in pro IL-1 beta. So in theory, both Catalog #s AF-201-NA and MAB601 should detect the pro form of IL-1 beta. The results would depend on whether the pro form is abundantly present in the sample type being evaluated.

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TGF-beta 1/1.2 Antibody
By Anonymous on 06/28/2017
Application: B/N Sample Tested: MRC-5 human embryonic lung fibroblast cell line Species: Human
Block/Neutralize TGF-beta 1/1.2 Antibody AF-101-NA