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Sleeping Beauty Transposase Antibody

Catalog #: AF2798
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2798
AF2798-SP
Product Details
Citations (9)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sleeping Beauty Transposase Antibody Summary

Specificity
Detects Sleeping Beauty Transposase in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant Sleeping Beauty Transposase
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Sleeping Beauty Transposase
Immunohistochemistry
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of transgenic mouse intestine expressing Sleeping Beauty transposase subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sleeping Beauty Transposase

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired‑like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).

References
  1. Ivics, Z. et al. (1997) Cell 91:501.
Alternate Names
SB Transposase

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Citations for Sleeping Beauty Transposase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Transposon Mutagenesis Reveals RBMS3 Silencing as a Promoter of Malignant Progression of BRAFV600E-Driven Lung Tumorigenesis
    Authors: Aria Vaishnavi, Joseph Juan, Maebh Jacob, Christopher Stehn, Eric E. Gardner, Michael T. Scherzer et al.
    Cancer Research
  2. Engineered Sleeping Beauty transposase redirects transposon integration away from genes
    Authors: C Miskey, L Kesselring, I Querques, G Abrusán, O Barabas, Z Ivics
    Nucleic Acids Research, 2022-03-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Targeting CD33 in Chemoresistant AML Patient-Derived Xenografts by CAR-CIK Cells Modified with an Improved SB Transposon System
    Authors: MC Rotiroti, C Buracchi, S Arcangeli, S Galimberti, MG Valsecchi, VM Perriello, T Rasko, G Alberti, CF Magnani, C Cappuzzell, F Lundberg, A Pande, G Dastoli, M Introna, M Serafini, E Biagi, Z Izsvák, A Biondi, S Tettamanti
    Mol. Ther., 2020-05-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition
    Nucleic Acids Res, 2016-12-01;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
    Authors: F Voigt, L Wiedemann, C Zuliani, I Querques, A Sebe, L Mátés, Z Izsvák, Z Ivics, O Barabas
    Nat Commun, 2016-03-30;7(0):11126.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  7. Transposon mutagenesis identifies genetic drivers of Braf(V600E) melanoma.
    Authors: Mann M, Black M, Jones D, Ward J, Yew C, Newberg J, Dupuy A, Rust A, Bosenberg M, McMahon M, Print C, Copeland N, Jenkins N
    Nat Genet, 2015-04-13;47(5):486-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. A transposon-based analysis of gene mutations related to skin cancer development.
    Authors: Quintana R, Dupuy A, Bravo A, Casanova M, Alameda J, Page A, Sanchez-Viera M, Ramirez A, Navarro M
    J Invest Dermatol, 2012-07-26;133(1):239-48.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  9. Whole-body sleeping beauty mutagenesis can cause penetrant leukemia/lymphoma and rare high-grade glioma without associated embryonic lethality.
    Authors: Collier LS, Adams DJ, Hackett CS, Bendzick LE, Akagi K, Davies MN, Diers MD, Rodriguez FJ, Bender AM, Tieu C, Matise I, Dupuy AJ, Copeland NG, Jenkins NA, Hodgson JG, Weiss WA, Jenkins RB, Largaespada DA
    Cancer Res., 2009-10-20;69(21):8429-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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