Simple Plex Human BMP-2 Cartridge

Catalog #: SPCKB-PS-000245 Datasheet / COA / SDS

Simple Plex Human BMP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000245
SPCKA-PS-000245
SPCKC-PS-000245

Control Products Available

894946: Simple Plex Control for Human BMP-2

Simple Plex Cartridge Kit for 72 samples, 1 analyte for use with Human Plasma / Serum.

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human BMP-2 Cartridge Summary

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

8.36 pg/mL

Assay Range

19.7 - 30,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human BMP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human BMP-2 in serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) control was Each tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	24
Mean (pg/mL)	170	8191	174	8201
Standard Deviation	12.8	636	16.9	541
CV%	7.5	7.8	9.7	6.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	95	87-107
Heparin Plasma (n=4)	93	83-106
Serum (n=4)	94	88-98

Linearity

Samples containing and/or spiked with high concentrations of human BMP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human BMP-2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-2

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name:

Bone Morphogenetic Protein 2

Entrez Gene IDs:

650 (Human); 12156 (Mouse); 29373 (Rat)

Alternate Names:

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC