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Sheep IgG (H+L) APC-conjugated Antibody

Catalog #: F0127
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0127
Detection of Sheep IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sheep IgG (H+L) APC-conjugated Antibody Summary

Species Reactivity
Sheep
Specificity
Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Sheep IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Sheep IgG Primary Antibody by Flow Cytometry View Larger

Detection of Sheep IgG Primary Antibody by Flow Cytometry Human peripheral blood granulocytes were stained with Sheep Anti-Human Fce RIa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6678, filled histogram) or isotype control antibody (Catalog # 5-001-A), open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) at 10 µL/106cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Sheep IgG (H+L) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Deep mutational scanning reveals functional constraints and antigenic variability of Lassa virus glycoprotein complex
    Authors: Carr, CR;Crawford, KHD;Murphy, M;Galloway, JG;Haddox, HK;Matsen, FA;Andersen, KG;King, NP;Bloom, JD;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells, Transfected Whole Cells
    Applications: Flow Cytometry
  2. Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer
    Authors: VA Stark, COB Facey, L Opdenaker, JZ Fields, BM Boman
    bioRxiv : the preprint server for biology, 2023-02-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. U3-1402 sensitizes HER3-expressing tumors to PD-1 blockade by immune activation
    Authors: K Haratani, K Yonesaka, S Takamura, O Maenishi, R Kato, N Takegawa, H Kawakami, K Tanaka, H Hayashi, M Takeda, N Maeda, T Kagari, K Hirotani, J Tsurutani, K Nishio, K Doi, M Miyazawa, K Nakagawa
    J. Clin. Invest., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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