Recombinant Zebrafish BMP-2a Protein

Catalog #: 111-BM
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
111-BM-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
111-BM-010

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Zebrafish BMP-2a Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 100-400 ng/mL.

Source

E. coli-derived zebrafish BMP-2a protein
Gln272-Arg386, with an N-terminal Met

Accession #

AAI63048.1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

13 kDa (monomer)

Product Datasheets

111-BM (with carrier)

Product Datasheet

COA

SDS

111-BM/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

111-BM

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

111-BM/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BMP-2a

BMP-2 is one of at least 20 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily (1, 2). BMPs were originally indentified as protein regulators of cartilage and bone formation. However, they are also involved in embryogenesis and morphogenesis of various tissues and organs. BMPs regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse, and rat BMP-2 are 100% identical, while mature human BMP-2 and zebrafish BMP-2a are 85% identical. Zebrafish have another homolog of BMP-2a, BMP-2b, which is 88% identical to BMP-2a in the mature region and corresponds to the swirl mutant. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates (3). Biologically active BMP-2a is a disulfide-linked homodimer of the carboxy-terminal 115 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2a have been shown to be mediated by the formation of hetero-ogliomeric complexes of type I and type II serine/threonine kinase receptors (4). In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type I’s can bind BMP-2 with high affinity in the absence of BMP receptor type II.

References

Ryoo, H.M. et al. (2006) Gene 366:51.
Chen, D. et al. (2004) Growth Factors 22:233.
Martinez-Barbera, J. et al. (1997) Gene 198:53.
Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.

Long Name

Bone Morphogenetic Protein 2a

Entrez Gene IDs

30631 (Zebrafish)

Alternate Names

BMP2a; BMP-2a

Related Research Areas

Citation for Recombinant Zebrafish BMP-2a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1