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Recombinant Mouse IFN-alpha 4 (Mammalian) Protein

Catalog #: 12115-1
3 Citations Datasheet / COA / SDS
Newer Version Available: 10259-IF
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Catalog # Availability Size / Price Qty
12115-1
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha 4 (Mammalian) Protein Summary

Product Specifications

Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Familletti et al. (1981) "A Convenience and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology , Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394] with the exception that EMCV rather than VSV was used as the challenge. The activity was determined relative to a lab standard of Mu-IFN-alpha A which was calibrated to the NIH Murine IFN-alpha standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol Meth. 306:1]
Source

Murine (C57/BI) DNA expressed in mammalian cells
Accession # NM_010504
Accession #
NM_010504
Predicted Molecular Mass
19.5 kDa

Product Datasheets

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12115-1

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

12115-1

Formulation Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 4

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name
Interferon alpha 4
Entrez Gene IDs
3441 (Human); 15967 (Mouse)
Alternate Names
IFNA4; IFNalpha 4; IFN-alpha 4; IFN-alpha-4; IFN-alpha4a; INFA4; interferon alpha-4; Interferon alpha-4B; Interferon alpha-76; Interferon alpha-M1; interferon, alpha 4; MGC142200

Citations for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape
    Authors: Cao, Y;Wu, J;Hu, Y;Chai, Y;Song, J;Duan, J;Zhang, S;Xu, X;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. IRF3-binding lncRNA-ISIR strengthens interferon production in viral infection and autoinflammation
    Authors: J Xu, P Wang, Z Li, Z Li, D Han, M Wen, Q Zhao, L Zhang, Y Ma, W Liu, M Jiang, X Zhang, X Cao
    Cell Reports, 2021-11-02;37(5):109926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TRIM38 Negatively Regulates TLR3/4-Mediated Innate Immune and Inflammatory Responses by Two Sequential and Distinct Mechanisms.
    Authors: Hu M, Xie X, Yang Q, Liao C, Ye W, Lin H, Shu H
    J Immunol, 2015-09-21;195(9):4415-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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