Recombinant Mouse IFN-alpha 4 Protein, CF

Name: Recombinant Mouse IFN-alpha 4 Protein, CF
Brand: R&D Systems
SKU: 10259-IF
Rating: 5 (1 reviews)

Catalog #: 10259-IF
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10259-IF-010
10259-IF-050

Recombinant Mouse IFN-alpha 4 Protein Bioactivity

2 Images

All IFN-alpha 4 Proteins and Enzymes

Product Details

Citations (1)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha 4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <60 pg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 4 protein
Leu23-Glu186

Accession #

P07351

N-terminal Sequence
Analysis

Leu23

Predicted Molecular Mass

19 kDa

SDS-PAGE

17-25 kDa, under reducing conditions

Product Datasheets

10259-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10259-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IFN-alpha/IFNA4 (Catalog # 10259-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <60 pg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IFN-alpha 4 (Catalog # 10259-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa.

Reconstitution Calculator

Background: IFN-alpha 4

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly (7).

References

Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
Fung, K.Y. et al. (2013) Science 339:1088.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
van Pesch, V. et al. (2004) J. Virol. 78:8219.
Yeow, W.S. et al. (1998) J. Immunol. 15:2932.
Gerlach, N. et al. (2009) European J. Immunol. 39:136.

Long Name

Interferon alpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Alternate Names

IFNA4; IFNalpha 4; IFN-alpha 4; IFN-alpha-4; IFN-alpha4a; INFA4; interferon alpha-4; Interferon alpha-4B; Interferon alpha-76; Interferon alpha-M1; interferon, alpha 4; MGC142200

Related Research Areas

Interferons

Citation for Recombinant Mouse IFN-alpha 4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cancer cell-derived type I interferons instruct tumor monocyte polarization
Authors: D Kwart, J He, S Srivatsan, C Lett, J Golubov, EM Oswald, P Poon, X Ye, J Waite, AG Zaretsky, S Haxhinasto, E Au-Yeung, NT Gupta, J Chiu, C Adler, S Cherravuru, E Malahias, N Negron, K Lanza, A Coppola, M Ni, H Song, Y Wei, GS Atwal, L Macdonald, NS Oristian, W Poueymirou, V Jankovic, M Fury, I Lowy, AJ Murphy, MA Sleeman, B Wang, D Skokos
Cell Reports, 2022-12-06;41(10):111769.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay