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Recombinant Mouse BMP-8a Protein, CF

Catalog #: 7540-BP
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7540-BP-025
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BMP-8a Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED50 for this effect is 0.5-5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BMP-8a protein
Ala261-His399
Accession #
P34821
N-terminal Sequence
Analysis
Ala261
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15.7 kDa (monomer)
SDS-PAGE
19 kDa, reducing conditions

Product Datasheets

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7540-BP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7540-BP

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-8a

BMP‑8, also known as osteogenic protein 2 (OP‑2), was first isolated from a hippocampal library in a screen to identify relatives of BMP‑7/OP‑1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF‑ beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2‑4). BMP‑8a and BMP‑8b, produced from separate genes, share 98% amino acid sequence identity in human but only 74% in mouse within the mature regions. Mouse BMP‑8a is synthesized as a 399 aa precursor protein that is cleaved between Arg260 and Ala261 to release the C‑terminal mature protein. Mature mouse BMP‑8a shares 90% aa sequence identity with mature human BMP‑8a and ‑8b. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5‑7). In the mouse it cooperates with BMP‑7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10‑12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

References
  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  4. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  5. Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
  6. Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
  7. Zhao, G.-Q. et al. (1998) Development 125:1103.
  8. Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
  9. Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
  10. van der Horst, G. et al. (2002) Bone 31:661.
  11. Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
  12. Paic, F. et al. (2009) Bone 45:682.
  13. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
Long Name
Bone Morphogenetic Protein 8a
Entrez Gene IDs
353500 (Human); 12163 (Mouse)
Alternate Names
BMP-8a; bone morphogenetic protein 8a; Op2; Osteogenic Protein 2

Citations for Recombinant Mouse BMP-8a Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Metabolic memory of ?9-tetrahydrocannabinol exposure in pluripotent stem cells and primordial germ cells-like cells
    Authors: Verdikt, R;Armstrong, AA;Cheng, J;Hwang, YS;Clark, AT;Yang, X;Allard, P;
    eLife
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Examining the Developmental Trajectory of an in Vitro Model of Mouse Primordial Germ Cells following Exposure to Environmentally Relevant Bisphenol A Levels
    Authors: SKT Ooi, H Jiang, Y Kang, P Allard
    Environmental health perspectives, 2021-09-29;129(9):97013.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Reconstitution of male germline cell specification from mouse embryonic stem cells using defined factors in vitro
    Authors: N Li, W Ma, Q Shen, M Zhang, Z Du, C Wu, B Niu, W Liu, J Hua
    Cell Death Differ., 2019-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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