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Recombinant Human NT-4 Protein

Catalog #: 268-N4
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
268-N4-005/CF
268-N4-025/CF

With Carrier

Catalog # Availability Size / Price Qty
268-N4-005
268-N4-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NT-4 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.3‑3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-4 protein
Gly81-Ala210
Accession #
P34130
N-terminal Sequence
Analysis
Gly81
Predicted Molecular Mass
14 kDa

Product Datasheets

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268-N4 (with carrier)

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268-N4/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

268-N4

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

268-N4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NT-4

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1-3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48-52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8-10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8-10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14-16).

References
  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.
Long Name
Neurotrophin 4
Entrez Gene IDs
4909 (Human)
Alternate Names
GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF4; NTF5; NTF5NT5

Citations for Recombinant Human NT-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome
    Authors: Y Tian, W Wang, S Lautrup, H Zhao, X Li, PWN Law, ND Dinh, EF Fang, HH Cheung, WY Chan
    Nature Communications, 2022-09-16;13(1):5456.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice
    Authors: D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt
    Cell Reports, 2021-12-21;37(12):110152.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
    Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
    Cells, 2020-08-21;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Cell Culture
  4. Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction
    Authors: N Michel, P Narayanan, O Shomroni, M Schmidt
    Cell Rep, 2020-07-21;32(3):107912.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mapping developmental trajectories and subtype diversity of normal and glaucomatous human retinal ganglion cells by single-cell transcriptome analysis
    Authors: P Teotia, M Niu, I Ahmad
    Stem Cells, 2020-06-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019-08-01;14(0):6117-6131.
    Species: Mouse
    Sample Types: Nanoparticles
    Applications: In Vivo
  7. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function.
    Authors: Royo NC, Lebold D, Magge SN, Chen I, Hauspurg A, Cohen AS, Watson DJ
    Neuroscience, 2007-08-02;148(2):359-70.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
    Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
    Chem. Biol., 2006-05-01;13(5):549-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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