Recombinant Human Follistatin-like 1 Protein, CF
Recombinant Human Follistatin-like 1 Protein, CF Summary
Product Specifications
Met1-Ile308, with a C-terminal Asp and 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1694-FN
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 200 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Follistatin-like 1/FSTL1
Follistatin‑like 1 (FSL1 or FSTL1), also known as FRP (follistatin‑related protein), Flik (follistatin‑like), and TSC‑36 (TGF‑ beta 1‑stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM‑40/SPARC/Osteonectin family (1‑3). The human FSL‑1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine‑rich Follistatin (EGF- and kazal‑like) domain, two apparently non‑functional EF‑hand calcium‑binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSL1 shares 94%, 95%, 98% and 99% aa identity with mouse, rat, bovine and equine FSL1, respectively. FSL1 was first identified as a TGF‑ beta ‑induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In vitro, FSTL1 interacts directly with BMP‑4, while in vivo, it regulates BMP‑4 signaling during normal lung development, as demonstrated by lethal respiratory failure in mice deleted for FSTL1 (6). In humans, FSL1 is a common rheumatoid arthritis auto‑antigen (2). It is reported to be either pro‑inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (7‑10). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (11, 12). Cardiac and circulating FSL1 is generally increased in conditions such as heart failure and acute coronary syndrome (12‑14). FSL1 also appears to be a tumor suppressor, showing down‑regulated expression in many human cancers (4, 15, 16). In vitro, it slows proliferation and MMP‑dependent migration, and increases FAS‑dependent apoptosis of tumor cell lines (15).
- Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
- Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
- Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
- Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
- Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
- Geng, Y et al. (2011) Proc. Natl. Acad. Sci. USA 108:7058.
- Clutter, S.D. et al. (2009) J. Immunol. 182:234.
- Miyamae, T. et al. (2006) J. Immunol. 177:4758.
- Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
- Tanaka, M. et al. (2003) Int. Immunol. 15:71.
- Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
- Oshima, Y. et al. (2008) Circulation 117:3099.
- Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
- Widera, C. et al. (2009) Clin. Chem. 55:1794.
- Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
- Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Citations for Recombinant Human Follistatin-like 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
The Journal of Biological Chemistry, 2022-05-04;0(0):102010.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival
Authors: M Wu, Y Ding, N Wu, J Jiang, Y Huang, F Zhang, H Wang, Q Zhou, Y Yang, W Zhuo, L Teng
American journal of cancer research, 2021-03-01;11(3):712-728.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Follistatin-like 1 protects against hypoxia-induced pulmonary hypertension in mice
Authors: W Zhang, W Wang, J Liu, J Li, J Wang, Y Zhang, Z Zhang, Y Liu, Y Jin, J Li, J Cao, C Wang, W Ning, J Wang
Sci Rep, 2017-03-31;7(0):45820.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A holistic approach to dissecting SPARC family protein complexity reveals FSTL-1 as an inhibitor of pancreatic cancer cell growth
Sci Rep, 2016-11-25;6(0):37839.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epicardial FSTL1 reconstitution regenerates the adult mammalian heart.
Authors: Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P
Nature, 2015-09-16;525(7570):479-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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