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Recombinant Human Cerberus 1 Protein, CF

Catalog #: 9515-CB Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9515-CB-025
Recombinant Human Cerberus 1 Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Cerberus 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Cerberus 1 (Catalog # 9515-CB) is coated at 2 µg/mL, Recombinant Human Nodal (Catalog # 3218-ND) binds with an ED50 = 0.4-2.4 ng/mL.
Source
Trichoplusia ni, T. ni (baculovirus)-derived human Cerberus 1 protein
Human Cerberus 1
(Thr18-Ala267)
Accession # O95813		HIEGRGGGSGGGSGGGSHHHHHHHHHH
N-terminusC-terminus
Accession #
O95813
N-terminal Sequence
Analysis
Thr18
Predicted Molecular Mass
31 kDa
SDS-PAGE
36-41 kDa, reducing conditions

Product Datasheets

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9515-CB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9515-CB

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Cerberus 1 Protein Bioactivity View Larger

When Recombinant Human Cerberus 1 (Catalog # 9515-CB) is coated at 2 µg/mL, Recombinant Human Nodal (Catalog # 3218-ND) binds with an ED50 = 0.4-2.4 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature human Cerberus 1 shares 67% and 68% amino acid (aa) sequence identity with mouse and rat Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24%-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

References
    1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
    2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
    3. Biben, C. et al. (1998) Dev. Biol. 194:135.
    4. Piccolo, S. et al. (1999) Nature 397:707.
    5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
    6. Tavares, A.T. et al. (2007) Development 134:2051.
    7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
    8. Bouwmeester, T. et al. (1996) Nature 382:595.
    9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
    10. Glinka, A. et al. (1997) Nature 389:517.
    11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
    12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
    13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Entrez Gene IDs
9350 (Human); 12622 (Mouse)
Alternate Names
CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951

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