Recombinant Human BMP-2 GMP Protein, CF

Catalog #: 355-GMP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
355-GMP-010
355-GMP-050

Recombinant Human BMP-2 GMP Protein Bioactivity

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Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-2 GMP Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED₅₀ for this effect is 40-200 ng/mL.
The specific activity of recombinant human BMP-2 is >5.0 x 10⁵ units/mg, which is calibrated against the human BMP-2 WHO Standard (NIBSC code: 93/574).

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-2 protein
Gln283-Arg396
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Accession #

P12643.1

N-terminal Sequence
Analysis

Gln-Ala-Lys-His-Lys-Gln-Arg-Lys-Arg-Leu

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

13 kDa (monomer)

SDS-PAGE

15-16, kDa, reducing conditions

Product Datasheets

355-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

355-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human BMP-2 (Catalog # 355-GMP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 40-200 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP BMP-2.

SDS-PAGE

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1 μg/lane of GMP-grade Recombinant Human BMP-2 (Catalog # 355-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 15 kDa and 30 kDa, respectively.

Reconstitution Calculator

Background: BMP-2

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

References

Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
Chen, D. et al. (2004) Growth Factors 22:233.
Wozney, J. et al. (1988) Science 242:1528.
Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269.
Sebald, W. et al. (2004) Biol. Chem. 385:697.
De Luca, F. et al. (2001) Endocrinology 142:430.
Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
Ryoo, H.-M. et al. (2006) Gene 366:51.
Kramer, J. et al. (2000) Mech. Dev. 92:193.
Li, X. et al. (2008) Atherosclerosis January 5 epub.
Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
Kano, Y. et al. (2005) Endocrinology 146:5332.
Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

Long Name

Bone Morphogenetic Protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Alternate Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material testing and vendor qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration schedules using a computerized calibration program
Facility maintenance, safety programs and pest control
Material review process for variances
Monitoring of stability over product shelf-life

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
Host Cell Protein testing performed by ELISA
Mycoplasma testing by ribosomal RNA hybridization assay

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Product Specific Notices

END USER TERMS OF USE OF PRODUCT

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