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Recombinant Human BMP-2/BMP-6 Heterodimer Protein

Catalog #: 7145-BP
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7145-BP-010/CF

With Carrier

Catalog # Availability Size / Price Qty
7145-BP-010
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Product Details
Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-2/BMP-6 Heterodimer Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.

The ED50 for this effect is 4-20 ng/mL.

Source
E. coli-derived human BMP-2/BMP-6 Heterodimer protein
Human BMP-2
(Ala284 - Arg396), with an N-terminal Met
Accession # P12643
Human BMP-6
(Gln382 - His513), with an N-terminal Met
Accession # P22004
N-terminus C-terminus
Accession #
P12643 (BMP-2) & P22004 (BMP-6)
N-terminal Sequence
Analysis
Ala284 (BMP-2) & Met (BMP-6)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
12.8 kDa (BMP-2) & 15 kDa (BMP-6)
SDS-PAGE
11 kDa & 14 kDa, reducing conditions

Product Datasheets

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7145-BP (with carrier)

Product Datasheet
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7145-BP/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7145-BP

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7145-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-6 Heterodimer

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, and BMP-2 are members of the BMP family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (1 - 4). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (5). Human BMP-6 is synthesized as a 513 aa precursor protein that contains a 20 aa signal sequence, a 354 aa prosegment, and a 139 aa mature region (6). BMP prosegments are removed by proteolysis, enabling the glycosylated 18 kDa mature BMPs to form active disulfide-linked homodimers or heterodimers (1, 2). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2, and mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. They share 48% aa sequence identity with each other. Both BMP-2 and BMP-6 induce osteogenic and chondrogenic differentiation in mesenchymal stem cells (4). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (7, 8). BMP-2/6 heterodimers also show increased activity at inducing trophoectodermal and endodermal differentiation of embryonic stem cells compared to either homodimer (9).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  3. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  4. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  5. Wozney, J. et al. (1988) Science 242:1528.
  6. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  7. Isaacs, M.J. et al. (2010) Mol. Endocrinol. 24:1469.
  8. Israel, D.I. et al. (1996) Growth Factors 13:291.
  9. Valera, E. et al. (2010) PLoS ONE 5:e11167.
Long Name
Bone Morphogenetic Protein 2/6
Entrez Gene IDs
650 (Human)
Alternate Names
BMP-2/BMP-6 Heterodimer

Citations for Recombinant Human BMP-2/BMP-6 Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
    Authors: A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers
    Int J Mol Sci, 2020-04-14;21(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue
    Authors: E Toyoda, M Sato, T Takahashi, M Maehara, Y Nakamura, G Mitani, T Takagaki, K Hamahashi, M Watanabe
    Regen Ther, 2018-11-20;10(0):17-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7
    Authors: LD Loozen, A Vanderstee, AH Kragten, FC Öner, WJ Dhert, MC Kruyt, J Alblas
    Eur Cell Mater, 2018-03-28;35(0):195-208.
    Applications: ELISA (Standard)

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