Recombinant Human BAMBI/NMA Fc Chimera Protein, CF Summary
Product Specifications
| Human BAMBI/NMA (Glu27-Ala152) Accession # Q13145 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
921-BM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: BAMBI/NMA
BAMBI (BMP and Activin membrane-bound inhibitor), also called NMA (non-metastatic gene A) is a type I transmembrane protein that shares sequence homology with the TGF‑ beta type I receptor family and functions as a decoy receptor (1). The 260 amino acid (aa) human BAMBI protein contains a 20 aa signal sequence, a 132 aa extracellular domain (ECD) with one potential N‑glycosylation site, a transmembrane domain and a cytoplasmic domain. The human BAMBI ECD shares 91%, 93%, 94%, 94%, 92%, 92% and 92% aa sequence identity with mouse, rat, canine, porcine, equine, ovine and bovine BAMBI, respectively. BAMBI differs from the
TGF-beta RI family by having a short cytoplasmic region that lacks the kinase domain (1, 2). It competes with type I receptors and forms heterodimers with type II receptors (2). During development, BAMBI is prominent in gastrulation, neurulation, and development of teeth and bones, and is often co‑expressed with BMP family members (1, 2, 4, 6). It antagonizes Activin and TGF‑ beta signaling, and may increase the dynamic range of BMPs to aid in BMP gradient effectiveness (4, 6). In the adult, it modulates processes such as adipogenesis, response to arterial injury, and tumor/tumor stroma promotion by TGF-beta (1, 3, 7, 9‑11). Although it is not expressed in other normal skin cells, it is highly expressed in melanocytes and may be inversely correlated with metastatic potential in melanomas (3). BAMBI is induced by TGF‑ beta and Wnt signaling, and cooperates with inhibitory Smad6 and Smad7 to inhibit TGF‑ beta signaling (5). In contrast, it promotes Wnt signaling (8).
- Knight, C. et al. (2001) J. Dent. Res. 80:1895.
- Onichtchouk, D. et al. (1999) Nature 401:480.
- Degen, W.G. et al. (1996) Int. J. Cancer 65:460.
- Paulsen, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:10202.
- Yan, X. et al. (2009) J. Biol. Chem. 284:30097.
- Montero, J.A. et al. (2008) Dev. Biol. 321:343.
- Guillot, N. et al. (2013) PLoS ONE 8:e58550.
- Lin, Z. et al. (2008) J. Biol. Chem. 283:33053.
- Luo, X. et al. (2012) Diabetes 61:124.
- Shangguan, L. et al. (2012) Stem Cells 30:2810.
- Sekiya, T. et al. (2004) J. Biol. Chem. 279:6840.
Citation for Recombinant Human BAMBI/NMA Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability.
Authors: Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B
Nat Commun, 2015-01-12;6(0):5999.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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