Rat TfR (Transferrin R) Antibody

Catalog #: MAB10079 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10079-100
MAB10079-SP

Detection of TfR (Transferrin R) in PC‑12 Rat Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TfR (Transferrin R) Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat TfR in Flow Cytometry.

Source

Monoclonal Mouse IgG_2A Clone # OX26

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

PHA activated rat lymphocytes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TfR (Transferrin R) in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat TfR (Transferrin R) Monoclonal Antibody (Catalog # MAB10079, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Goat anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR (Transferrin R)

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR. sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency. Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production. Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling. TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses.

Long Name

Transferrin Receptor

Entrez Gene IDs

7037 (Human); 22042 (Mouse); 64678 (Rat)

Alternate Names

CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR