Rat IL-7R alpha/CD127 PE-conjugated Antibody

Catalog #: FAB8484P
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8484P-025
FAB8484P-100

Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry.

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Rat IL-7R alpha/CD127 PE-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL-7 R alpha /CD127 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 717519

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
Glu21-Asp239
Accession # NP_001099888

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD4 APC-conjugated Antibody and either (A) Mouse Anti-Rat IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB8484P) or (B) Mouse IgG_2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-7R alpha/CD127

IL-7 R alpha (IL-7 Receptor alpha), also known as CD127, is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8⁺ T cells, DN thymocytes, pre-B cells, Th1 CD4⁺ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP Receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4⁺ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III Fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.

Long Name

Interleukin 7 Receptor alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Alternate Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Rat IL-7R alpha/CD127 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Innate lymphoid cell characterization in the rat and their correlation to gut commensal microbes
Authors: A Sudworth, FM Segers, B Yilmaz, NC Guslund, AJ Macpherson, E Dissen, SW Qiao, M Inngjerdin
European Journal of Immunology, 2022-02-13;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry