Rat IL-1 beta /IL-1F2 Biotinylated Antibody Summary
Val117-Ser268
Accession # Q63264
Applications
Rat IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL-1 beta /IL-1F2 was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Rat IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF501) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Rat IL-1 beta /IL-1F2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Acute stress induces the rapid and transient induction of caspase-1, gasdermin D and release of constitutive IL-1 beta protein in dorsal hippocampus
Authors: Matthew G. Frank, Michael V. Baratta, Kaixin Zhang, Isabella P. Fallon, Mikayleigh A. Pearson, Guozhen Liu et al.
Brain, Behavior, and Immunity
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Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infect. Immun., 2008-06-09;76(8):3577-86.
Species: Rat
Sample Types: BALF
Applications: ELISA Development -
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006-09-29;181(1):57-67.
Species: Rat
Sample Types: Plasma
Applications: ELISA Development
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This biotinylated antibody paired well with the unbiotinylated version, AF501, in a sandwich ELISA to measure rat IL-1b in serum samples.
