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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Catalog #: F0105B
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0105B
Detection of Rat IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rat IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rat IgG Primary Antibody by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 Monoclonal Antibody (Catalog # MAB25351, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) at 10 µL/106cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Development of anti-human mesothelin-targeted chimeric antigen receptor (CAR) messenger RNA (mRNA)-transfected peripheral blood lymphocytes (CARMA-hMeso) for ovarian cancer therapy
    Authors: CF Hung, X Xu, L Li, Y Ma, Q Jin, A Viley, C Allen, P Natarajan, R Shivakumar, MV Peshwa, LA Emens
    Hum. Gene Ther., 2018-04-02;0(0):.
    Species: Human, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  2. Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model
    Authors: K Dai, Y Huang, Z Chen, X Sun, L Yang, Y Jiang
    Eur. J. Immunol., 2018-01-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells.
    Authors: Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA
    J. Immunol., 2012-06-13;189(2):956-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus.
    Authors: Gourzi P, Leonova T, Papavasiliou FN
    Immunity, 2006-06-01;24(6):779-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients
    Authors: Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al.
    Frontiers in Oncology
  6. A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts
    Authors: Artur Filipe Rodrigues, Catarina Rebelo, Susana Simões, Cristiana Paulo, Sónia Pinho, Vítor Francisco et al.
    Advanced Science

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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody
By Pinar Aksoy on 12/08/2022
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

HCT116 cells were stained with unconjugated MSLN antibody or isotype control. Then the secondary antibody was applied at 10 µL/10^6 cells. Pink is iso control and blue is the MSLN stained sample.