Rat CXCR2/IL-8RB PE-conjugated Antibody

Catalog #: FAB8110P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8110P

Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry.

2 Images

All CXCR2/IL-8RB Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCR2/IL-8RB PE-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCR2/IL-8 RB in ELISA. Stains rat CXCR2/IL-8 RB transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 866614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR2/IL-8 RB
Accession # P35407

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry. Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB8110P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of CXCR2/IL‑8 RB in HEK Human Cell Line Transfected with Rat CSCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB8110P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Long Name

Interleukin 8 Receptor B

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Alternate Names

CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B