Rat CCL19/MIP-3 beta Antibody

Catalog #: MAB7800 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7800
MAB7800-SP

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Rat CCL19/MIP‑3 beta Antibody.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCL19/MIP-3 beta Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCL19/MIP‑3 beta in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 866202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat CCL19/MIP‑3 beta
Gly26-Ser108
Accession # NP_001102131

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 μg/ml in the presence of 25 ng/mL Recombinant Rat CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Rat CCL19/MIP‑3 beta Antibody. Recombinant Rat CCL19/MIP-3 beta (Catalog # 7800-M3) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB7800). The ND₅₀ is typically 0.4-2.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

CCL19 (also known as Mip-3 beta, ELC, CK beta -11, Scya19 and Exodus-3) is a 9 kDa secreted member of the CC family of chemotactic cytokines (1-4). Cells known to express CCL19 are limited in number, and include activated monocytes (5), stromal cells in nodal T zones (6), CD8⁺ dendritic cells (DC) (6), vascular endothelial cells (7), visceral smooth muscle and mast cells (8), and thymic stromal cells (9). Rat CCL19 is synthesized as a precursor that is 1108 amino acids (aa) in length. Mature rat CCL19 shares 72% and 89% aa sequence identity with human and mouse CCL19, respectively. CCL19 is known to bind to CCR7 (3, 4), CCLR2/CRAM (L-CCR in rodent) (10, 11) and CCX-CKR (12), with the last two receptors representing scavenger, or chemokine-sink receptors.

References

Sharma, M. (2010) Crit. Rev. Biotechnol. 30:1.
Blanchet, X. et al. (2012) Front. Immunol. 3:175.
Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
Luther, S.A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12694.
Alt, C. et al. (2002) Eur. J. Immunol. 32:2133.
Kaur, D. et al. (2006) Am. J. Respir. Crit. Care Med. 174:1179.
Takamura, K. et al. (2007) J. Immunol. 179:5897.
Leick, M. et al. (2009) Immunology 129:536.
Yoshimura, T. & J.J. Oppenheim (2011) Exp. Cell Res. 317:674.
Comerford, I. et al. (2006) Eur. J. Immunol. 36:1904

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names

beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19