Rat B7-1/CD80 Antibody

Catalog #: MAB1214 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1214
MAB1214-SP

All B7-1/CD80 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat B7-1/CD80 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat B7‑1/CD80 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human B7-1, recombinant mouse (rm) B7-1, rmB7-2, recombinant rat B7-2, rmB7-H1, rmB7-H2, rmB7-H3, or rmPD-L2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 210408

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat B7‑1/CD80
Ile39-Gln248
Accession # AAB60503

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat B7‑1/CD80 Fc Chimera (Catalog # 1214-B7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

It is widely accepted that T cells require a signal induced by the engagement of the T cell receptor and a co-stimulatory signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co-stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3 and PD-L2 (1). B7-1 is expressed on activated B cells, activated T cells, mature dendritic cells and macrophages (2). T cells express two different receptors capable of binding B7-1, CD28 and CTLA-4. CTLA-4 binds to B7-1 with a 20 - 100 fold higher affinity than CD28. Engagement of CD28 by B7-1 increases T cell proliferation and IL-2 production and enhances the immune response (3). In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response (4). Rat B7-1 is a 320 amino acid (aa) protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Rat and human B7-1 share 47% amino acid identity.

References

Coyle, A.J. and J-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
Azuma, M. et al. (1992) Nature 366:76.
Gimmi, C.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88:6575.
Walunas, T.L. et al. (1994) Immunity 1:405.

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Alternate Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80