Proteome Profiler Rat XL Cytokine Array
Proteome Profiler Rat XL Cytokine Array Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.
Key Benefits
- Detects 79 rat cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Rat XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Rat XL Cytokine Array nitrocellulose membranes spotted with 79 different antibodies to rat cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Rat XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
| Simultaneously detect the levels of these proteins in a single sample. | ||
| Adiponectin | Galectin-3 | LIX |
| CCL2/JE/MCP-1 | G-CSF | MAG/Siglec-4a |
| CCL3/CCL4/MIP-1α/β | GDF-15 | MMP-2 |
| CCL5/RANTES | GM-CSF | MMP-3 |
| CCL11/Eotaxin | Hepassocin | MMP-9 |
| CCL17/TARC | HGF | Neprilysin/CD10 |
| CCL20/MIP-3α | ICAM-1/CD54 | NOV/CCN3 |
| CCL21/6Ckine | IFN-γ | NT-3 |
| CCL22/MDC | IGF-I | NT-4 |
| Clusterin | IGFBP-2 | Osteopontin (OPN) |
| CNTF | IGFBP-3 | Osteoprotegerin/TNFRSF11B |
| CX3CL1/Fractalkine | IGFBP-5 | PDGF-BB |
| CXCL2/GROβ/MIP-2/CINC-3 | IGFBP-6 | Pref-1/DLK1/FA1 |
| CXCL7/Thymus Chemokine-1 | IL-1α/IL-1F1 | Prolactin |
| Cyr61/CCN1 | IL-1β/IL-1F2 | RAGE |
| Cystatin C | IL-1ra/IL-1F3 | RBP4 |
| DPPIV/CD26 | IL-2 | Resistin |
| EGF | IL-3 | RGM-A |
| EG-VEGF | IL-4 | SCF |
| Endostatin | IL-6 | Serpin E1/PAI-1 |
| Fetuin A | IL-13 | TIM-1/KIM-1/HAVCR |
| FGF acidic | IL-17A | TNF-α |
| FGF-7 | IL-22 | TWEAK/TNFSF12 |
| FGF-21 | Jagged 1 | VCAM-1/CD106 |
| Fibulin 3 | LIF | VEGF |
| Flt-3 Ligand | Lipocalin-2/NGAL | WISP-1/CCN |
| Galectin-1 | ||
Assays for Analytes represented in the Rat XL Cytokine Array
| Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development System |
| Adiponectin | RRP300 | DY3100-05 |
| CCL2/JE/MCP-1 | MJE00 | |
| CCL3/CCL4/MIP-1α/β | ||
| CCL5/RANTES | ||
| CCL11/Eotaxin | ||
| CCL17/TARC | ||
| CCL20/MIP-3α | DY540 | |
| CCL21/6Ckine | ||
| CCL22/MDC | ||
| Clusterin | ||
| CNTF | DY557 | |
| CX3CL1/Fractalkine | DY537 | |
| CXCL2/GROβ/MIP-2/CINC-3 | RCN300 | DY525 |
| CXCL7/Thymus Chemokine-1 | DY1116 | |
| Cyr61/CCN1 | ||
| Cystatin C | ||
| DPPIV/CD26 | ||
| EGF | DY3214 | |
| EG-VEGF | ||
| Endostatin | ||
| Fetuin A | ||
| FGF acidic | ||
| FGF-7 | ||
| FGF-21 | ||
| Fibulin 3 | ||
| Flt-3 Ligand | ||
| Galectin-1 | ||
| Galectin-3 | ||
| G-CSF | ||
| GDF-15 | MGD150 | |
| GM-CSF | DY518 | |
| Hepassocin | ||
| HGF | ||
| ICAM-1/CD54 | RIC100 | DY583 |
| IFN-γ | PRIF00 | DY585 |
| IGF-I | ||
| IGFBP-2 | ||
| IGFBP-3 | ||
| IGFBP-5 | ||
| IGFBP-6 | ||
| IL-1α/IL-1F1 | RRA00 | DY500 |
| IL-1β/IL-1F2 | PRLB00 | DY501 |
| IL-1ra/IL-1F3 | ||
| IL-2 | PR2000 | DY502 |
| IL-3 | ||
| IL-4 | R4000 | DY504 |
| IL-6 | PR6000B | DY506 |
| IL-13 | ||
| IL-17A | ||
| IL-22 | ||
| Jagged 1 | DY599 | |
| LIF | ||
| Lipocalin-2/NGAL | DY3508 | |
| LIX | DY543 | |
| MAG/Siglec-4a | DY538 | |
| MMP-2 | ||
| MMP-3 | ||
| MMP-9 | RMP900 | DY8174-05 |
| Neprilysin/CD10 | ||
| NOV/CCN3 | ||
| NT-3 | ||
| NT-4 | ||
| Osteopontin (OPN) | ||
| Osteoprotegerin/TNFRSF11B | ||
| PDGF-BB | MBB00 | DY8464-05 |
| Pref-1/DLK1/FA1 | ||
| Prolactin | ||
| RAGE | DY1616 | |
| RBP4 | ||
| Resistin | ||
| RGM-A | ||
| SCF | ||
| Serpin E1/PAI-1 | ||
| TIM-1/KIM-1/HAVCR | ||
| TNF-α | PRTA00 | DY510 |
| TWEAK/TNFSF12 | ||
| VCAM-1/CD106 | ||
| VEGF | RRV00 | DY564 |
| WISP-1/CCN |
Specifications
Product Datasheets
Scientific Data
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1A: NR8383 normal rat lung cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).
View Larger
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1B: IEC-18 rat intestinal epithelial cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).
View Larger
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2A: Lysates from IEC-18 rat intestinal epithelial cells were left untreated or treated with 100 ng/mL PMA for 24 hours (200 μg lysate, 5 minute exposure.
View Larger
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2B: Lysates from NR8383 normal rat lung cells were left untreated or treated with 5 μg/mL zymosan for 24 hours (200 μg lysate, 5 minute exposure).
Citations for Proteome Profiler Rat XL Cytokine Array
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Time-dependent effects of BRAF-V600E on cell cycling, metabolism, and function in engineered myocardium
Authors: Strash, N;DeLuca, S;Janer Carattini, GL;Chen, Y;Wu, T;Helfer, A;Scherba, J;Wang, I;Jain, M;Naseri, R;Bursac, N;
Science advances 2024-01-26
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CXCR2-Blocking Has Context-Sensitive Effects on Rat Glioblastoma Cell Line Outgrowth (S635) in an Organotypic Rat Brain Slice Culture Depending on Microglia-Depletion (PLX5622) and Dexamethasone Treatment
Authors: Falter, J;Lohmeier, A;Eberl, P;Stoerr, EM;Koskimäki, J;Falter, L;Rossmann, J;Mederer, T;Schmidt, NO;Proescholdt, M;
International journal of molecular sciences 2023-11-27
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Authors: H Ma, WS Siu, CM Koon, XX Wu, X Li, W Cheng, WT Shum, CB Lau, CK Wong, PC Leung
International Journal of Molecular Sciences, 2023-01-10;24(2):. 2023-01-10
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Neuroinflammation in the anterior cingulate cortex: the potential supraspinal mechanism underlying the mirror-image pain following motor fiber injury
Authors: QY Li, SX Chen, JY Liu, PW Yao, YW Duan, YY Li, Y Zang
Journal of Neuroinflammation, 2022-06-20;19(1):162. 2022-06-20
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Rat prostate tumors induce DNA synthesis in remote organs
Authors: S Halin Berg, M Lundholm, A Nordstrand, A Bergh
Scientific Reports, 2022-05-12;12(1):7908. 2022-05-12
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Subtoxic cell responses to silica particles with different size and shape
Authors: M Kersting, M Olejnik, N Rosenkranz, K Loza, M Breisch, A Rostek, G Westphal, J Bünger, N Ziegler, A Ludwig, M Köller, C Sengstock, M Epple
Scientific Reports, 2020-12-09;10(1):21591. 2020-12-09
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Bidirectional Enhancement of Cell Proliferation Between Iron Oxide Nanoparticle-Labeled Mesenchymal Stem Cells and Choroid Plexus in a Cell-Based Therapy Model of Ischemic Stroke
Authors: MR Wu, CH Lee, JK Hsiao
Int J Nanomedicine, 2020-11-19;15(0):9181-9195. 2020-11-19
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Comparative study of intracavernous pressure and cavernous pathology after bilateral cavernous nerve crushing and resection in rats
Authors: M Li, YM Yuan, BC Yang, SJ Gu, HX Li, ZC Xin, D Fang, RL Guan
Asian J. Androl., 2020-11-01;0(0):. 2020-11-01
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Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles
Authors: FF Chen, MM Wang, WW Xia, DY Peng, L Han
Chin J Nat Med, 2020-08-01;18(8):620-627. 2020-08-01
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A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation
Authors: N Goshi, RK Morgan, PJ Lein, E Seker
J Neuroinflammation, 2020-05-11;17(1):155. 2020-05-11
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The Effect of Cardiogenic Factors on Cardiac Mesenchymal Cell Anti-Fibrogenic Paracrine Signaling and Therapeutic Performance
Authors: JS Heidel, AG Fischer, XL Tang, G Sadri, WJ Wu, CR Moisa, H Stowers, N Sandella, M Wysoczynsk, S Uchida, JB Moore Iv
Theranostics, 2020-01-01;10(4):1514-1530. 2020-01-01
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Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal
Authors: EM Hill, RM Esper, A Sen, BR Simon, MN Aslam, Y Jiang, MK Dame, SD McClintock, JA Colacino, Z Djuric, MS Wicha, WL Smith, DE Brenner
J. Nutr. Biochem., 2019-05-24;71(0):45-53. 2019-05-24
FAQs
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
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