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Rat IL-1 beta/IL-1F2 DuoSet ELISA

Catalog #: DY501
82 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY501
DY501-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Rat IL-1 beta / IL-1F2 ELISA Standard Curve
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Product Details
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Citations (82)
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Reviews (4)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Rat IL-1 beta/IL-1F2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat IL-beta. The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

 

Scientific Data

N/A Rat IL-1 beta / IL-1F2 ELISA Standard Curve View Larger

Rat IL-1 beta / IL-1F2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1 alpha, IL-1 beta, and IL-1ra, plus IL-18, IL-33, and IL-1F5-10. IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions (1). IL-1 is not produced by unstimulated cells of healthy mice with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. In response to inflammatory agents, infections, or microbial endotoxins, however, a dramatic increase in the production of IL-1 by macrophages and various other cell types is observed. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases (2-5). 

 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa (6, 7). Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response (2, 8). Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide (9-11), but evidence suggests that these factors can be secreted by non-classical pathways (12, 13). A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity (14). The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form (13, 15). Both unprocessed and mature forms of IL-1 beta are exported from the cell. 
 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes (16, 17). The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells (18). The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 amino acids (aa), whereas the type I receptor has a 217 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function (19). The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction (20). IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 (21, 22). Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines (23, 24). In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses (25).

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Rat IL-1 beta/IL-1F2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

82 Citations: Showing 1 - 10
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  1. MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effects
    Authors: Anttila, JE;Mattila, OS;Liew, HK;Mätlik, K;Mervaala, E;Lindholm, P;Lindahl, M;Lindsberg, PJ;Tseng, KY;Airavaara, M;
    Acta neuropathologica communications
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats
    Authors: Duan, R;Hou, J;Wang, X;Huang, Z;Cao, H;Hu, J;Peng, Q;Duan, H;Wang, Q;Chen, X;
    Brain sciences
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Potential Benefits of Epidermal Growth Factor for Inhibiting Muscle Degrative Markers in Rats with Alcoholic Liver Damage
    Authors: Xiao, Q;Chen, YH;Chen, YL;Chien, YS;Hsieh, LH;Shirakawa, H;Yang, SC;
    International journal of molecular sciences
    Species: Rat
    Sample Types: Tissue Homogenates
  4. Targeting TGF-beta/periostin signaling by sesamol ameliorates pulmonary fibrosis and improves lung function and survival
    Authors: SK Tirunavall, M Kuncha, R Sistla, SB Andugulapa
    The Journal of nutritional biochemistry, 2023-03-21;0(0):109294.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Efficacy and Safety Evaluation of Mometasone Furoate in Treating Ocular Inflammation
    Authors: NA Lage, MRB de Paiva, DV Vasconcelo, RR Machado, SL Fialho, A Silva-Cunh
    Pharmaceutics, 2023-01-05;15(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. Triptolide Attenuates Muscular Inflammation and Oxidative Stress in a Delayed-Onset Muscle Soreness Animal Model
    Authors: CC Hsu, CC Tsai, PY Ko, TH Kwan, MY Liu, PT Wu, IM Jou
    International Journal of Environmental Research and Public Health, 2022-12-12;19(24):.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. High-mobility Group Box 1 Contributes to Hypoxic-Ischemic Brain Damage by Facilitating Imbalance of Microglial Polarization through RAGE-PI3K/Akt Pathway in Neonatal Rats
    Authors: Y Sun, X Zhu, K Zhu, J Yu, L Cheng, M Hei
    International Journal of Medical Sciences, 2022-11-21;19(14):2093-2103.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. Mitochondrial protective effects caused by the administration of mefenamic acid in sepsis
    Authors: D Dominguini, M Michels, LB Wessler, EL Streck, T Barichello, F Dal-Pizzol
    Journal of Neuroinflammation, 2022-11-04;19(1):268.
    Species: Rat
    Sample Types: Tissue Homogenates
  9. Effect of citronellol on oxidative stress, neuroinflammation and autophagy pathways in an in vivo model of Parkinson's disease
    Authors: RL Jayaraj, S Azimullah, KA Parekh, SK Ojha, R Beiram
    Heliyon, 2022-11-03;8(11):e11434.
    Species: Rat
    Sample Types: Tissue Homogenates
  10. Glia from the central and peripheral nervous system are differentially affected by paclitaxel chemotherapy via modulating their neuroinflammatory and neuroregenerative properties
    Authors: I Klein, J Boenert, F Lange, B Christense, MK Wassermann, MHJ Wiesen, DN Olschewski, M Rabenstein, C Müller, HC Lehmann, GR Fink, M Schroeter, MA Rueger, SU Vay
    Frontiers in Pharmacology, 2022-11-02;13(0):1038285.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. Enriched environment causes epigenetic changes in hippocampus and improves long-term cognitive function in sepsis
    Authors: E Córneo, M Michels, M Abatti, A Vieira, RC Gonçalves, FF Gabriel, H Borges, A Goulart, N da Silva M, D Dominguini, R Varela, S Valvassori, F Dal-Pizzol
    Scientific Reports, 2022-07-07;12(1):11529.
    Species: Rat
    Sample Types: Tissue Homogenates
  12. Microglia Depletion from Primary Glial Cultures Enables to Accurately Address the Immune Response of Astrocytes
    Authors: M Van Zeller, AM Sebastião, CA Valente
    Biomolecules, 2022-05-04;12(5):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  13. Anti-inflammatory role of GM1 and other gangliosides on microglia
    Authors: D Galleguill, Q Wang, N Steinberg, A Zaidi, G Shrivastav, K Dhami, GC Daskhan, EN Schmidt, Z Dworsky-Fr, F Giuliani, M Churchward, C Power, K Todd, A Taylor, MS Macauley, S Sipione
    Journal of Neuroinflammation, 2022-01-06;19(1):9.
    Species: Rat
    Sample Types: Cell Culture Supernates
  14. Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
    Authors: MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim
    International Journal of Environmental Research and Public Health, 2022-01-04;19(1):.
    Species: Rat
    Sample Types: BALF
  15. Prenatal administration of IL-1Ra attenuate the neurodevelopmental impacts following non-pathogenic inflammation during pregnancy
    Authors: ME Brien, K Hughes, S Girard
    Scientific Reports, 2021-12-03;11(1):23404.
    Species: Rat
    Sample Types: Placenta
  16. Moderate Physical Exercise Activates ATR2 Receptors, Improving Inflammation and Oxidative Stress in the Duodenum of 2K1C Hypertensive Rats
    Authors: ACA da Silva, JS Severo, BLB Dos Santos, PHM Mendes, LMS Nobre, AP de Oliveir, FCS Ferreira, JVR Medeiros, RC Lima-Junio, A Havt, RC Palheta-Ju, AA Dos Santos, M Tolentino
    Frontiers in Physiology, 2021-10-14;12(0):734038.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage
    Authors: Q Xiao, YH Chen, SA Pratama, YL Chen, H Shirakawa, HC Peng, SC Yang
    Nutrients, 2021-08-14;13(8):.
    Species: Rat
    Sample Types: Tissue Homogenates
  18. Programming of intestinal homeostasis in male rat offspring after maternal exposure to chlorpyrifos and/or to a high fat diet
    Authors: M Guibourden, H El Khayat, N Djekkoun, H Khorsi-Cau, V Bach, PM Anton, J Gay-Quéhei
    Scientific Reports, 2021-06-01;11(1):11420.
    Species: Rat
    Sample Types: Plasma
  19. Inflammatory Cytokine Profile and Plasticity of Brain and Spinal Microglia in Response to ATP and Glutamate
    Authors: SJB Jesudasan, SJ Gupta, MA Churchward, KG Todd, IR Winship
    Frontiers in Cellular Neuroscience, 2021-04-06;15(0):634020.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production
    Authors: VGB Brito, MS Patrocinio, MCL Sousa, AEA Barreto, SCT Frasnelli, VS Lara, CF Santos, SHP Oliveira
    PLoS ONE, 2021-03-04;16(3):e0247372.
    Species: Rat
    Sample Types: Tissue Homogenates
  21. Differential and paradoxical roles of new-generation antidepressants in primary astrocytic inflammation
    Authors: JH He, RP Liu, YM Peng, Q Guo, LB Zhu, YZ Lian, BL Hu, HH Fan, X Zhang, JH Zhu
    Journal of Neuroinflammation, 2021-02-18;18(1):47.
    Species: Rat
    Sample Types: Cell Culture Supernates
  22. Small intestine remodeling in male Goto-Kakizaki rats
    Authors: JNB Pereira, GM Murata, FT Sato, AR Marosti, CRO Carvalho, R Curi
    Physiological Reports, 2021-02-01;9(3):e14755.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. The Poststroke Peripheral Immune Response Is Differentially Regulated by Leukemia Inhibitory Factor in Aged Male and Female Rodents
    Authors: SM Davis, LA Collier, SJ Messmer, KR Pennypacke
    Oxidative Medicine and Cellular Longevity, 2020-12-10;2020(0):8880244.
    Species: Rat
    Sample Types: Tissue Homogenates
  24. &alpha-Bisabolol, a Dietary Bioactive Phytochemical Attenuates Dopaminergic Neurodegeneration through Modulation of Oxidative Stress, Neuroinflammation and Apoptosis in Rotenone-Induced Rat Model of Parkinson's disease
    Authors: H Javed, MFN Meeran, S Azimullah, L Bader Eddi, VD Dwivedi, NK Jha, S Ojha
    Biomolecules, 2020-10-08;10(10):.
    Species: Rat
    Sample Types: Tissue Homogenates
  25. Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage
    Authors: YL Chen, H Shirakawa, NS Lu, HC Peng, Q Xiao, SC Yang
    J. Nutr. Biochem., 2020-09-11;86(0):108491.
    Species: Rat
    Sample Types: Cell Culture Supernates
  26. Perillyle alcohol and Quercetin ameliorate monocrotaline-induced pulmonary artery hypertension in rats through PARP1-mediated miR-204 down-regulation and its downstream pathway
    Authors: S Rajabi, H Najafipour, S Jafarineja, S Joukar, A Beik, M Iranpour, Z Kordestani
    BMC Complement Med Ther, 2020-07-13;20(1):218.
    Species: Rat
    Sample Types: Lung Tissue
  27. Progesterone Attenuates Stress-Induced NLRP3 Inflammasome Activation and Enhances Autophagy following Ischemic Brain Injury
    Authors: C Espinosa-G, F Atif, S Yousuf, I Sayeed, GN Neigh, DG Stein
    Int J Mol Sci, 2020-05-26;21(11):.
    Species: Rat
    Sample Types: Cell Supernatants
  28. Hepcidin-to-Ferritin Ratio Is Decreased in Astrocytes With Extracellular Alpha-Synuclein and Iron Exposure
    Authors: J Cui, X Guo, Q Li, N Song, J Xie
    Front Cell Neurosci, 2020-03-10;14(0):47.
    Species: Rat
    Sample Types: Cell Culture Supernates
  29. Acute sleep deprivation during pregnancy in rats: Rapid elevation of placental and fetal inflammation and kynurenic acid
    Authors: AM Baratta, NR Kanyuch, CA Cole, H Valafar, J Deslaurier, A Pocivavsek
    Neurobiol Stress, 2019-12-14;12(0):100204.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Protective effect of Platymiscium floribundum Vog. in tree extract on periodontitis inflammation in rats
    Authors: JMO Freire, HV Chaves, AH Teixeira, LHT de Sousa, IR Pinto, JJDN Costa, NA de Sousa, KMA Pereira, VCC Girão, VCS Ferreira, JEÁ Dos Santos, MAS Lima, ATA Pimenta, RC Montenegro, MEA de Moraes, VPT Pinto, GC Filho, MM Bezerra
    PLoS ONE, 2019-11-04;14(11):e0223800.
    Species: Rat
    Sample Types: Gingival Fluid
  31. The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity
    Authors: P Siriarchav, MC Kruger, MR Miller, HS Tian, FM Wolber
    Nutrients, 2019-07-15;11(7):.
    Species: Rat
    Sample Types: Plasma
  32. Interleukin-1? Promotes Schwann Cells De-Differentiation in Wallerian Degeneration via the c-JUN/AP-1 Pathway
    Authors: G Chen, X Luo, W Wang, Y Wang, F Zhu, W Wang
    Front Cell Neurosci, 2019-07-09;13(0):304.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  33. A diet including xanthan gum triggers a pro-inflammatory response in Wistar rats inoculated with Walker 256 cells
    Authors: AB Silva Risc, NIP Neto, K Gascho, M Carnier, DA de Miranda, FP Silva, VT Boldarine, M Seelaender, EB Ribeiro, LM Oyama, CM Oller do N
    PLoS ONE, 2019-06-18;14(6):e0218567.
    Species: Rat
    Sample Types: Tissue Homogenates
  34. Time dependent dual effect of anti-inflammatory treatments on sarin-induced brain inflammation: suggested role of prostaglandins
    Authors: S Chapman, E Grauer, R Gez, I Egoz, S Lazar
    Neurotoxicology, 2019-05-13;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  35. Hyperbilirubinemia in Gunn Rats is Associated with Decreased Inflammatory Response in LPS-Mediated Systemic Inflammation
    Authors: P Valaskova, A Dvorak, M Lenicek, K Zizalova, N Kutinova-C, J Zelenka, M Cahova, L Vitek, L Muchova
    Int J Mol Sci, 2019-05-09;20(9):.
    Species: Rat
    Sample Types: Serum
  36. Exosomes derived from umbilical cord mesenchymal stem cells reduce microglia-mediated neuroinflammation in perinatal brain injury
    Authors: G Thomi, D Surbek, V Haesler, M Joerger-Me, A Schoeberle
    Stem Cell Res Ther, 2019-03-21;10(1):105.
    Species: Rat
    Sample Types: Tissue Homogenates
  37. BRD4 inhibition attenuates inflammatory response in microglia and facilitates recovery after spinal cord injury in rats
    Authors: J Wang, J Chen, H Jin, D Lin, Y Chen, X Chen, B Wang, S Hu, Y Wu, Y Wu, Y Zhou, N Tian, W Gao, X Wang, X Zhang
    J. Cell. Mol. Med., 2019-02-26;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  38. Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
    Authors: CA Barboza, AR Fukushima, N Carrozzi, JF Machi, PMM Dourado, CT Mostarda, MC Irigoyen, L Nathanson, M Morris, EC Caperuto, B Rodrigues
    Sci Rep, 2019-02-21;9(1):2481.
    Species: Rat
    Sample Types: Plasma
  39. Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats
    Authors: TR Falcão, CAO Rodrigues, AA de Araújo, CACX de Medeiro, LAL Soares, MRA Ferreira, RC Vasconcelo, RF de Araújo, MLD de Sousa L, GCB Guerra
    BMC Complement Altern Med, 2019-02-12;19(1):47.
    Species: Rat
    Sample Types: Synovial Fluid
  40. Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
    Authors: WS Tan, P Arulselvan, SF Ng, CN Mat Taib, MN Sarian, S Fakurazi
    BMC Complement Altern Med, 2019-01-17;19(1):20.
    Species: Rat
    Sample Types: Tissue Homogenates
  41. Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils
    Authors: WMT Kuwabara, CNF Yokota, R Curi, TC Alba-Loure
    Sci Rep, 2018-12-03;8(1):17534.
    Species: Rat
    Sample Types: BALF
  42. Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats
    Authors: HF Filippini, PA Scalzilli, KM Costa, RDS Freitas, MM Campos
    PLoS ONE, 2018-11-12;13(11):e0207411.
    Species: Rat
    Sample Types: Serum
  43. Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
    Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
    Cell Rep, 2018-10-16;25(3):677-689.e4.
    Species: Rat
    Sample Types: Serum
  44. Leukemia inhibitory factor modulates the peripheral immune response in a rat model of emergent large vessel occlusion
    Authors: SM Davis, LA Collier, ED Winford, CC Leonardo, CT Ajmo, EA Foran, TJ Kopper, JC Gensel, KR Pennypacke
    J Neuroinflammation, 2018-10-15;15(1):288.
    Species: Rat
    Sample Types: Tissue Homogenates
  45. Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
    Authors: N Shologu, M Scully, JG Laffey, D O'Toole
    Int J Mol Sci, 2018-09-30;19(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  46. Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia?reperfusion
    Authors: B Wang, H Xu, J Li, HM Gao, YH Xing, Z Lin, HJ Li, YQ Wang, SH Cao
    Mol Med Rep, 2018-09-14;0(0):.
    Species: Rat
    Sample Types: Serum
  47. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury
    Authors: S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd
    J Neuroinflammation, 2018-03-20;15(1):90.
    Species: Rat
    Sample Types: Cell Culture Supernates
  48. Cortical stimulation in conscious rats controls joint inflammation
    Authors: GS Bassi, L Ulloa, VR Santos, F Del Vecchi, P Delfino-Pe, GJ Rodrigues, JA Castania, FQ Cunha, HC Salgado, TM Cunha, N Garcia-Cai, A Kanashiro
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018-03-06;0(0):.
    Species: Rat
    Sample Types: Synovial Fluid
  49. P2X7receptor antagonism ameliorates renal dysfunction in a rat model of sepsis
    Authors: N Arulkumara, ML Sixma, S Pollen, E Ceravola, E Jentho, M Prendecki, PS Bass, FWK Tam, RJ Unwin, M Singer
    Physiol Rep, 2018-03-01;6(5):.
    Species: Rat
    Sample Types: Tissue Homogenates
  50. Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
    Authors: YW Chien, HC Peng, YL Chen, MH Pai, HY Wang, HL Chuang, SC Yang
    Mediators Inflamm., 2017-12-13;2017(0):5801768.
    Species: Rat
    Sample Types: Tissue Homogenates
  51. The role of Src & ERK1/2 kinases in inspiratory resistive breathing induced acute lung injury and inflammation
    Authors: D Toumpanaki, V Vassilakop, I Sigala, P Zacharatos, I Vraila, V Karavana, S Theocharis, T Vassilakop
    Respir. Res., 2017-12-13;18(1):209.
    Species: Rat
    Sample Types: Tissue Homogenates
  52. Cholinergic Stimulation Improves Oxidative Stress and Inflammation in Experimental Myocardial Infarction
    Authors: OC Bezerra, CM França, JA Rocha, GA Neves, PRM Souza, M Teixeira G, C Malfitano, TCA Loleiro, PM Dourado, S Llesuy, K de Angelis, MCC Irigoyen, L Ulloa, FM Consolim-C
    Sci Rep, 2017-10-20;7(1):13687.
    Species: Rat
    Sample Types: Tissue Homogenates
  53. ?9-Tetrahydrocannabinol Prevents Cardiovascular Dysfunction in STZ-Diabetic Wistar-Kyoto Rats
    Authors: RK Vella, DJ Jackson, AS Fenning
    Biomed Res Int, 2017-10-18;2017(0):7974149.
    Species: Rat
    Sample Types: Serum
  54. Carotid sinus nerve electrical stimulation in conscious rats attenuates systemic inflammation via chemoreceptor activation
    Authors: FM Santos-Alm, G Domingos-S, CA Meschiari, LC Fávaro, C Becari, JA Castania, A Lopes, TM Cunha, DJA Moraes, FQ Cunha, L Ulloa, A Kanashiro, GCSV Tezini, HC Salgado
    Sci Rep, 2017-07-24;7(1):6265.
    Species: Rat
    Sample Types: Plasma
  55. Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation
    Authors: LD Almeida Ju, AEV Quaglio, CAR de Almeida, LC Di Stasi
    World J. Gastroenterol., 2017-06-28;23(24):4369-4380.
    Species: Rat
    Sample Types: Tissue Homogenates
  56. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage
    Authors: CC Hsu, CL Lin, IM Jou, PH Wang, JS Lee
    Bone Joint Res, 2017-04-01;6(4):253-258.
    Species: Rat
    Sample Types: Tissue Homogenates
  57. Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
    Authors: S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau
    Sci Rep, 2017-01-20;7(0):40373.
    Species: Rat
    Sample Types: Tissue Homogenates
  58. Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
    Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
    Int J Mol Sci, 2017-01-18;18(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  59. The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury
    PLoS ONE, 2016-12-28;11(12):e0167677.
    Species: Rat
    Sample Types: Tissue Homogenates
  60. Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats
    Authors: Cláudia Herrera Tambeli
    J Pain, 2016-11-04;0(0):.
    Species: Rat
    Sample Types: Synovial Lavage Fluid
  61. Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
    Authors: Bruno Rodrigues
    Int. J. Cardiol., 2016-10-26;0(0):.
    Species: Rat
    Sample Types: Plasma
  62. Combination of methylprednisolone and rosiglitazone promotes recovery of neurological function after spinal cord injury
    Neural Regen Res, 2016-10-01;11(10):1678-1684.
    Species: Rat
    Sample Types: Tissue Homogenates
  63. Biodistribution and Clearance of TiO2 Nanoparticles in Rats after Intravenous Injection.
    Authors: Elgrabli D, Beaudouin R, Jbilou N, Floriani M, Pery A, Rogerieux F, Lacroix G
    PLoS ONE, 2015-04-24;10(4):e0124490.
    Species: Rat
    Sample Types: Serum
  64. S-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis.
    Authors: Skeff M, Brito G, de Oliveira M, Braga C, Cavalcante M, Baldim V, Holanda-Afonso R, Silva-Boghossian C, Colombo A, Ribeiro R, Moura-Neto V, Leitao R
    PLoS ONE, 2014-12-05;9(12):e113378.
    Species: Hamster
    Sample Types: Tissue Homogenates
  65. Muscle hyperalgesia induced by peripheral P2X3 receptors is modulated by inflammatory mediators.
    Authors: Schiavuzzo J, Teixeira J, Melo B, da Silva dos Santos D, Jorge C, Oliveira-Fusaro M, Parada C
    Neuroscience, 2014-11-20;285(0):24-33.
    Species: Rat
    Sample Types: Tissue Homogenates
  66. Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain.
    Authors: Baskar Jesudasan, Sam Josh, Todd, Kathryn, Winship, Ian R
    PLoS ONE, 2014-06-10;9(6):e99443.
    Species: Rat
    Sample Types: Cell Culture Supernates
  67. Dietary zinc deficiency fuels esophageal cancer development by inducing a distinct inflammatory signature.
    Authors: Taccioli, C, Chen, H, Jiang, Y, Liu, X P, Huang, K, Smalley, K J, Farber, J L, Croce, C M, Fong, L Y
    Oncogene, 2011-12-19;31(42):4550-8.
    Species: Rat
    Sample Types: Tissue Homogenates
  68. Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6.
    Authors: Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ
    Stem Cells, 2011-10-01;29(10):1572-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  69. Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction.
    Authors: Yamashita AS, Lira FS, Rosa JC, Paulino EC, Brum PC, Negrao CE, dos Santos RV, Batista ML, do Nascimento CO, Oyama LM, Seelaender M
    Cytokine, 2010-08-21;52(3):168-74.
    Species: Rat
    Sample Types: Tissue Homogenates
  70. TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae.
    Authors: Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J
    J. Neuroimmunol., 2010-03-03;221(1):42-5.
    Species: Rat
    Sample Types: Tissue Homogenates
  71. Comparative studies on the interplay of testosterone, estrogen and progesterone in collagen induced arthritis in rats.
    Authors: Ganesan K, Balachandran C, Manohar BM, Puvanakrishnan R
    Bone, 2008-06-10;43(4):758-65.
    Species: Rat
    Sample Types: Serum
  72. Augmentation of endogenous cannabinoid tone modulates lipopolysaccharide-induced alterations in circulating cytokine levels in rats.
    Authors: Roche M, Kelly JP, O'Driscoll M, O'Driscoll M, Finn DP
    Immunology, 2008-04-03;125(2):263-71.
    Species: Rat
    Sample Types: Plasma
  73. MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes.
    Authors: Fernandes A, Falcao AS, Silva RF, Brito MA, Brites D
    Eur. J. Neurosci., 2007-02-01;25(4):1058-68.
    Species: Rat
    Sample Types: Cell Culture Supernates
  74. Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model.
    Authors: Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG
    Eur. Respir. J., 2006-09-27;28(6):1236-44.
    Species: Rat
    Sample Types: BALF
  75. The influence of kainic acid on core temperature and cytokine levels in the brain.
    Authors: Oprica M, Spulber SD, Aronsson AF, Post C, Winblad B, Schultzberg M
    Cytokine, 2006-09-06;35(1):77-87.
    Species: Rat
    Sample Types: Tissue Homogenates
  76. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006-05-11;318(2):741-50.
    Species: Rat
    Sample Types: Tissue Homogenates
  77. Minocycline treatment prevents cavitation in rats after a cortical devascularizing lesion.
    Authors: Hua R, Walz W
    Brain Res., 2006-05-02;1090(1):172-81.
    Species: Rat
    Sample Types: Tissue Homogenates
  78. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006-02-10;96(6):1667-79.
    Species: Rat
    Sample Types: Cell Culture Supernates
  79. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006-02-08;96(6):1560-71.
    Species: Rat
    Sample Types: Tissue Homogenates
  80. Cardiomyocyte function after burn injury and lipopolysaccharide exposure: single-cell contraction analysis and cytokine secretion profile.
    Authors: Niederbichler AD, Westfall MV, Su GL, Donnerberg J, Usman A, Vogt PM, Ipaktchi KR, Arbabi S, Wang SC, Hemmila MR
    Shock, 2006-02-01;25(2):176-83.
    Species: Rat
    Sample Types: Cell Culture Supernates
  81. Cigarette smoke alters chromatin remodeling and induces proinflammatory genes in rat lungs.
    Authors: Marwick JA, Kirkham PA, Stevenson CS, Danahay H, Giddings J, Butler K, Donaldson K, MacNee W, Rahman I
    Am. J. Respir. Cell Mol. Biol., 2004-08-27;31(6):633-42.
    Species: Rat
    Sample Types: Tissue Homogenates
  82. A study of the biologic activity of trauma-hemorrhagic shock mesenteric lymph over time and the relative role of cytokines.
    Authors: Davidson MT, Deitch EA, Lu Q, Osband A, Feketeova E, Nemeth ZH, Hasko G, Xu DZ
    Surgery, 2004-07-01;136(1):32-41.
    Species: Rat
    Sample Types: Lymph

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Rat IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 08/01/2023
Sample Tested: Lung tissue
Rat IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 05/19/2023
Sample Tested: Lung tissue
Rat IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 02/11/2023
Sample Tested: Serum
Rat IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant

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Rat IL-1 beta/IL-1F2 DuoSet ELISA DY501