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Porcine/Equine CD14 Antibody

Catalog #: MAB4597
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4597
MAB4597-SP
Detection of CD14 in Porcine PBMCs by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine/Equine CD14 Antibody Summary

Species Reactivity
Porcine, Equine
Specificity
Detects porcine CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD14 and recombinant mouse CD14 is observed.
Source
Monoclonal Mouse IgG2B Clone # 433423
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine CD14
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine CD14
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD14 antibody in Porcine PBMCs antibody by Flow Cytometry. View Larger

Detection of CD14 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of CD14 antibody in Equine PBMCs antibody by Flow Cytometry. View Larger

Detection of CD14 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. It is a pattern recognition protein that acts as a receptor for lipopolysaccharide (LPS) and other microbial cell wall components. CD14 also occurs as a secreted protein. Mature porcine CD14 shares 70‑75% aa identity with human, cow, sheep, water buffalo and horse CD14 and 61‑62% aa identity with mouse and rat CD14.

Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Citations for Porcine/Equine CD14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Effect of the Clenbuterol-&beta2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen Species Production in Race Horses In Vitro
    Authors: O Witkowska-, R Pingwara, J Szczepania, A Winnicka
    Cells, 2021-04-17;10(4):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells
    Authors: L Abdelhamid, H Hussein, M Ghanem, N Eissa
    Res. Vet. Sci., 2017-05-05;114(0):225-232.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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