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Oligodendrocyte Marker O4 APC-conjugated Antibody

Catalog #: FAB1326A
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1326A
Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry.
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Oligodendrocyte Marker O4 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
Source
Monoclonal Mouse IgM Clone # O4
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Bovine brain corpus callosum white matter
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Oligodendrocyte Marker O4 antibody in Differentiated Rat Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry. Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 APC-conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Oligodendrocyte Marker O4

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Citation for Oligodendrocyte Marker O4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A comprehensive library of human transcription factors for cell fate engineering
    Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
    Nature Biotechnology, 2020-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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