NucA nuclease Antibody

Catalog #: MAB100631 Datasheet / COA / SDS

Detects Benzonase®. Benzonase® is a registered trademark of Merck KGaA Corporation. Bio-Techne Corporation and its affiliates, and their respective products and services, are not sponsored, endorsed, or approved by Merck KGaA Corporation

Catalog #	Availability	Size / Price	Qty
MAB100631-100
MAB100631-SP

Detection of NucA nuclease by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

NucA nuclease Antibody Summary

Specificity

Detects Benzonase^® Nuclease/NucA in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2404C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived S. marcescens recombinant Benzonase^® Nuclease/NucA
Met1-Asn266
Accession # P13717

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Recombinant NucA nuclease Protein

Simple Western

10 µg/mL

Recombinant NucA nuclease Protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100631) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of NucA nuclease by Simple Western^TM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100631). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NucA nuclease

Long Name

NucA nuclease

Entrez Gene IDs

23387471 (Bacterial)

Alternate Names

DcD; NucA nuclease

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

Benzonase is a registered trademark of Merck KGaA Corp.

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