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Myosin Heavy Chain Antibody

Catalog #: MAB4470
158 Citations 7 Reviews Datasheet / COA / SDS
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MAB4470
MAB4470-SP
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Myosin Heavy Chain in C2C12 Mouse Cell Line.
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Citations (158)
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Myosin Heavy Chain Antibody Summary

Specificity
Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
Source
Monoclonal Mouse IgG2B Clone # MF20
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chicken pectoralis-derived Myosin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Stains, C.I., et al. (2012) Chem Biol. 19:210.
 
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Myosin Heavy Chain antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Myosin Heavy Chain antibody in Human Skeletal Muscle by Immunohistochemistry (IHC-P). View Larger

Myosin Heavy Chain in Human Skeletal Muscle. Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Myosin Heavy Chain antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Myosin Heavy Chain in Mouse Skeletal Muscle. Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse Myosin Heavy Chain by Western Blot View Larger

Detection of Mouse Myosin Heavy Chain by Western Blot Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence Generation of DM1-iPSCs and their differentiation.(A) The strategy of our study: patient iPSCs were passed and differentiated at three different passage numbers into CMs or neurons giving 9 samples (left), or had a MyoD1 vector transfected and were differentiated into myocytes, giving 6 samples (right). The CTG repeat lengths were measured in each sample. (B) Six clones from three different DM1 patients expressed pluripotent stem cell markers (Oct3/4, Nanog and Sox2) in conventional PCR. beta -actin was used as a loading control. (C) Karyotypic analysis of undifferentiated iPSCS (Pt-1B). (D, left) Representative live image of CMs on day 20 (Pt-1B). A video clip is available in Supplementary Video 1. (D, right) FACS analysis of the CMs shown in the picture on the left. The X-axis indicates the percentage of cardiac troponin T (cTnT)-positive cells among the total number of CMs. The Y-axis indicates the autofluorescence of the CMs. (E) Representative immunostaining image of neurons on day 42 (Pt-1B). The left panel shows neurons that expressed Tyrosine Hydroxylase (TH) and Microtubule-associated protein 2 (Map2). The right panel shows neurons that expressed TH and Neuron-specific Class III beta -tubulin (TUJ1). (F) Representative immunostaining image of myocytes on day 7 (Pt-1B). The myocytes expressed Myosin Heavy Chain (MHC). Hoechst stains the nuclei. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep42522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Myosin Heavy Chain by Western Blot View Larger

Detection of Mouse Myosin Heavy Chain by Western Blot LiCl rescued the inhibitory effect of BAMBI siRNA on C2C12 myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (b) the western blot images of MyoD, MyHC, and GAPDH; (c) the protein expression of MyoD at 48 h and MyHC at 96 h; (d) immunofluorescence images of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (e) the populations of myotubes; (f) the differentiation index and (g) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Myosin Heavy Chain by Immunocytochemistry/Immunofluorescence Differentiated myotubes do not differ significantly between HD- and HF-derived skeletal muscle progenitor cells. (a) At day 7 after stimulation, myotubes were stained for the expression of MyHC with an antibody that recognizes the heavy chain of myosin II (MF20) and markers of slow MYH7 and fast MYH1/MYH2 fibers. Nuclei were labelled with DAPI (blue). Representative images are given for both HF- and HD-derived samples. Scale bars represent 50 μm. (b) Fusion coefficient is calculated as a percent of nuclei incorporated in MF20+ myotubes at day 7 after stimulation, and it does not differ between HD- and HF-derived samples. (c) mRNA expression analysis was performed for key markers of muscle development and metabolism for both HF- and HD-derived samples. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30719048), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Myosin Heavy Chain by Western Blot View Larger

Detection of Mouse Myosin Heavy Chain by Western Blot MiR-106a-5p inhibited the myogenic differentiation of C2C12 myoblasts. (A) Overexpression efficiency of miR-106a-5p 3 days (d) and 5 d post differentiation. NC: negative control; (B) The fluorescent microscopy images of C2C12 cells transfected with FAM-labeled miR-106a-5p agomir (×10). Scale bars = 500 μm; (C) Immunostaining for MyHC (red) and DAPI (blue) on 5 d post differentiation (×20). Scale bars = 100 μM; (D–F) The statistical results of differentiation index, fusion index and the populations of myotubes, respectively;1-3 indicates myotubes with 1, 2 or 3 nucleus, >4 indicates myotubes with 4 more nucleus; (G,H) The mRNA expression of MyoD, MyoG, MyHC on 3 d and 5 d post differentiation; (I,J) The mRNA expression of Myomarker and Myomixer 3 d and 5 d post differentiation; (K) The statistical results of MyoD, MyoG, MyHC proteins in Figure 2L; (L) Western blot analyzed for MyoD, MyoG, MyHC proteins 5 d post differentiation; (M) Protein levels of key molecules in PI3K-AKT pathway in C2C12 cells transfected with miR-106a-5p agomir or NC on 5 d post differentiation; (N) The statistical analysis of phosphorylated PI3K (p85 alpha ), AKT (sre473) and mTOR (ser2448). Data were presented as mean ± SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30004470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myosin Heavy Chain

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Alternate Names
DFNA4; FLJ13881; FLJ43092; KIAA2034DKFZp667A1311; MHC16; MYH14 variant protein; MYH17; MYHC; Myosin heavy chain 14; Myosin Heavy Chain; Myosin heavy chain, non-muscle IIc; myosin; myosin, heavy chain 14; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C

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Citations for Myosin Heavy Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

158 Citations: Showing 1 - 10
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  1. Preclinical testing of the glycogen synthase kinase-3 beta inhibitor tideglusib for rhabdomyosarcoma
    Authors: Narendra Bharathy, Matthew N. Svalina, Teagan P. Settelmeyer, Megan M. Cleary, Noah E. Berlow, Susan D. Airhart et al.
    Oncotarget
  2. Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts
    Authors: Dhananjay V. Deshmukh, Nils Pasquero, Gajraj Rathore, Joel Zvick, Ori Bar‐Nur, Jurg Dual et al.
    Bioengineering & Translational Medicine
  3. Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase
    Authors: Evelin Major, Ilka Keller, Dániel Horváth, István Tamás, Ferenc Erdődi, Beáta Lontay
    International Journal of Molecular Sciences
  4. The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
    Authors: SA Samant, A Kanwal, VB Pillai, R Bao, MP Gupta
    Sci Rep, 2017-09-19;7(1):11877.
  5. Filopodia powered by class x myosin promote fusion of mammalian myoblasts
    Authors: Hammers DW, Hart CC, Matheny MK et al.
    eLife
  6. ZAK beta is activated by cellular compression and mediates contraction‐induced MAP kinase signaling in skeletal muscle
    Authors: Cathrine Nordgaard, Anna Constance Vind, Amy Stonadge, Rasmus Kjøbsted, Goda Snieckute, Pedro Antas et al.
    The EMBO Journal
  7. p38 alpha Regulates Expression of DUX4 in a Model of Facioscapulohumeral Muscular Dystrophy
    Authors: L. Alejandro Rojas, Erin Valentine, Anthony Accorsi, Joseph Maglio, Ning Shen, Alan Robertson et al.
    Journal of Pharmacology and Experimental Therapeutics
  8. Laminin mimetic peptide nanofibers regenerate acute muscle defect
    Authors: Cagla Eren Cimenci, Gozde Uzunalli, Ozge Uysal, Fatih Yergoz, Ebru Karaca Umay, Mustafa O. Guler et al.
    Acta Biomaterialia
  9. Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis
    Authors: Aeyung Kim, Sang-Min Park, No Soo Kim, Haeseung Lee
    Antioxidants (Basel)
  10. HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathies
    Authors: Barbara Tedesco, Leen Vendredy, Elias Adriaenssens, Marta Cozzi, Bob Asselbergh, Valeria Crippa et al.
    Autophagy
  11. BAMBI Promotes C2C12 Myogenic Differentiation by Enhancing Wnt/ beta -Catenin Signaling
    Authors: Qiangling Zhang, Xin-E Shi, Chengchuang Song, Shiduo Sun, Gongshe Yang, Xiao Li
    International Journal of Molecular Sciences
  12. Anti-Fibrotic Effect of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells on Skeletal Muscle Cells, Mediated by Secretion of MMP-1
    Authors: Alee Choi, Sang Eon Park, Jang Bin Jeong, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu et al.
    International Journal of Molecular Sciences
  13. Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line
    Authors: Y Kitajima, Y Ono
    Skelet Muscle, 2018-08-24;8(1):26.
  14. A Systemically Administered Unconjugated Antisense Oligonucleotide Targeting DUX4 Improves Muscular Injury and Motor Function in FSHD Model Mice
    Authors: Tetsuhiro Kakimoto, Akira Ogasawara, Kiyoshi Ishikawa, Takashi Kurita, Kumiko Yoshida, Shuichi Harada et al.
    Biomedicines
  15. Lnc-GD2H Promotes Proliferation by Forming a Feedback Loop With c-Myc and Enhances Differentiation Through Interacting With NACA to Upregulate Myog in C2C12 Myoblasts
    Authors: Rui Chen, Si Lei, Yanling She, Shanyao Zhou, Huacai Shi, Cheng Li et al.
    Frontiers in Cell and Developmental Biology
  16. In vitro exercise model using contractile human and mouse hybrid myotubes
    Authors: Weijian Chen, Mazvita R. Nyasha, Masashi Koide, Masahiro Tsuchiya, Naoki Suzuki, Yoshihiro Hagiwara et al.
    Scientific Reports
  17. A Modified Pre-plating Method for High-Yield and High-Purity Muscle Stem Cell Isolation From Human/Mouse Skeletal Muscle Tissues
    Authors: Kiyoshi Yoshioka, Yasuo Kitajima, Narihiro Okazaki, Ko Chiba, Akihiko Yonekura, Yusuke Ono
    Frontiers in Cell and Developmental Biology
  18. Loss of tafazzin results in decreased myoblast differentiation in C2C12 cells: A myoblast model of Barth syndrome and cardiolipin deficiency
    Authors: Wenjia Lou, Christian A. Reynolds, Yiran Li, Jenney Liu, Maik Hüttemann, Michael Schlame et al.
    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
  19. MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIk3R1 and Modulating the PI3k/AkT Signaling.
    Authors: Li X, Zhu Y, Zhang H et al.
    Genes (Basel).
  20. Effectual Endeavors of Silk Protein Sericin against Isoproterenol Induced Cardiac Toxicity and Hypertrophy in Wistar Rats
    Authors: Farogh Ahsan, Tarique Mahmood, Tanveer A. Wani, Seema Zargar, Mohammed Haris Siddiqui, Shazia Usmani et al.
    Life (Basel)
  21. Endothelial protective genes induced by statin are mimicked by ERK5 activation as triggered by a drug combination of FTI-277 and GGTI-298
    Authors: Uyen B. Chu, Tyler Duellman, Sara J. Weaver, Yunting Tao, Jay Yang
    Biochimica et Biophysica Acta (BBA) - General Subjects
  22. Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss.
    Authors: Das S, Browne K D et al.
    Commun Biol
  23. Acute estradiol treatment reduces skeletal muscle protein breakdown markers in early- but not late-postmenopausal women
    Authors: Young-Min Park, Amy C. Keller, Shauna S. Runchey, Benjamin F. Miller, Wendy M. Kohrt, Rachael E. Van Pelt et al.
    Steroids
  24. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
    Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
    Sci Adv, 2019-05-08;5(5):eaau8857.
  25. Role of p53 in Cisplatin-Induced Myotube Atrophy
    Authors: Matsumoto C, Sekine H, Zhang N et al.
    International Journal of Molecular Sciences
  26. Preclinical Investigation of Alpinetin in the Treatment of Cancer-Induced Cachexia via Activating PPAR gamma
    Authors: Yujie Zhang, Yuxin Zhang, Yichen Li, Li Zhang, Shiying Yu
    Frontiers in Pharmacology
  27. The ensured proliferative capacity of myoblast in serum-reduced conditions with Methyl-beta -cyclodextrin
    Authors: Tomoka Katayama, Yuta Chigi, Daiji Okamura
    Frontiers in Cell and Developmental Biology
  28. HIF1 alpha -dependent mitophagy facilitates cardiomyoblast differentiation
    Authors: Jin-Feng Zhao, Catherine E. Rodger, George F. G. Allen, Simone Weidlich, Ian G. Ganley
    Cell Stress
  29. Water‐soluble dietary fiber alleviates cancer‐induced muscle wasting through changes in gut microenvironment in mice
    Authors: Tomoki Sakakida, Takeshi Ishikawa, Toshifumi Doi, Ryuichi Morita, Yuki Endo, Shinya Matsumura et al.
    Cancer Science
  30. Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.
    Authors: Park S, Choi Y, Jung N et al.
    Int. J. Mol. Med.
  31. Chondroitin sulfate E downregulates N-cadherin and suppresses myotube formation
    Authors: Fumi SATOH, Akihiro SUGIURA, Jiro TASHIRO, Yoshinao Z. HOSAKA, Katsuhiko WARITA
    Journal of Veterinary Medical Science
  32. Autophagy induction in the skeletal myogenic differentiation of human tonsil-derived mesenchymal stem cells
    Authors: Saeyoung Park, Yoonyoung Choi, Namhee Jung, Jieun Kim, Seiyoon Oh, Yeonsil Yu et al.
    International Journal of Molecular Medicine
  33. Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence
    Authors: Enis Kostallari, Yasmine Baba-Amer, Sonia Alonso-Martin, Pamela Ngoh, Frederic Relaix, Peggy Lafuste et al.
    Development
  34. The secretome of human dental pulp stem cells protects myoblasts from hypoxia induced injury via the Wnt/beta catenin pathway
    Authors: Zhang W, Yu L, Han X et al.
    Int. J. Mol. Med.
  35. Periostin Is Required for the Maintenance of Muscle Fibers during Muscle Regeneration
    Authors: Naoki Ito, Yuko Miyagoe-Suzuki, Shin’ichi Takeda, Akira Kudo
    International Journal of Molecular Sciences
  36. Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease
    Authors: Ricardo Ferrari, Bowen Xie, Edwyn Assaf, Kristin Morder, Melanie Scott, Hong Liao et al.
    Cells
  37. MiR-199-3p enhances muscle regeneration and ameliorates aged muscle and muscular dystrophy
    Authors: Masashi Fukuoka, Hiromi Fujita, Kosumo Numao, Yasuko Nakamura, Hideo Shimizu, Masayuki Sekiguchi et al.
    Communications Biology
  38. Elimination of Mutant mtDNA by an Optimized mpTALEN Restores Differentiation Capacities of Heteroplasmic MELAS-iPSCs
    Authors: Yahata N, Boda H, Hata R
    Molecular therapy. Methods & clinical development
  39. Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
    Authors: Yasuro Furuichi, Yuki Kawabata, Miho Aoki, Yoshitaka Mita, Nobuharu L. Fujii, Yasuko Manabe
    Frontiers in Cell and Developmental Biology
  40. Methionine availability influences essential H3K36me3 dynamics during cell differentiation
    Authors: Sun, Y;Ramesh, V;Wei, F;Locasale, JW;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Apabetalone, a Clinical-Stage, Selective BET Inhibitor, Opposes DUX4 Target Gene Expression in Primary Human FSHD Muscle Cells
    Authors: Sarsons, CD;Gilham, D;Tsujikawa, LM;Wasiak, S;Fu, L;Rakai, BD;Stotz, SC;Carestia, A;Sweeney, M;Kulikowski, E;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Diosgenin prevents dexamethasone-induced myotube atrophy in C2C12?cells
    Authors: Yoshioka, Y;Oishi, S;Onoda, K;Shibata, K;Miyoshi, N;
    Archives of biochemistry and biophysics
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, ICC
  43. Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regeneration
    Authors: Jo, SI;Park, YS;Chang, Y;Moon, JH;Lee, S;Lee, H;Kim, M;Kim, DY;Bae, S;Park, SY;Yun, H;You, JE;Im, M;Han, HJ;Kim, SY;Jin, DH;
    Biochemistry and biophysics reports
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  44. BRD4 isoforms have distinct roles in tumor progression and metastasis in embryonal rhabdomyosarcoma
    Authors: Das, D;Leung, JY;Tergaonkar, V;Loh, AHP;Chiang, CM;Taneja, R;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: ICC
  45. MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
    Authors: Cao, H;Du, T;Li, C;Wu, L;Liu, J;Guo, Y;Li, X;Yang, G;Jin, J;Shi, X;
    BMC genomics
    Species: Mouse
    Sample Types: Cell Lysates, Transfected Whole Cells
    Applications: Western Blot, ICC
  46. S100A8, S100A9 and S100A8/A9 heterodimer as novel cachexigenic factors for pancreatic cancer-induced cachexia
    Authors: Liao, WC;Chen, CT;Tsai, YS;Wang, XY;Chang, YT;Wu, MS;Chow, LP;
    BMC cancer
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  47. Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1
    Authors: Tao, W;Ouyang, Z;Liao, Z;Li, L;Zhang, Y;Gao, J;Ma, L;Yu, S;
    Cancers
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  48. Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz-Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles
    Authors: Y Yamashita, S Nakada, K Nakamura, H Sakurai, K Ohno, T Goto, Y Mabuchi, C Akazawa, N Hattori, E Arikawa-Hi
    Biomedicines, 2023-03-07;11(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IF
  49. Cardiolipin metabolism regulates expression of muscle transcription factor MyoD1 and muscle development
    Authors: L Vo, MW Schmidtke, NT Da Rosa-Ju, M Ren, M Schlame, ML Greenberg
    The Journal of Biological Chemistry, 2023-02-04;0(0):102978.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  50. Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence
    Authors: K Shintani-I, R Tsurumi, H Ikegaya
    PLoS ONE, 2023-01-17;18(1):e0280527.
    Species: Mouse
    Sample Types: Cell Lysates, Transfected Whole Cells
    Applications: ICC, Western Blot
  51. miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1
    Authors: L Li, X Zhang, H Yang, X Xu, Y Chen, D Dai, S Zhan, J Guo, T Zhong, L Wang, J Cao, H Zhang
    International Journal of Molecular Sciences, 2022-12-12;23(24):.
    Species: Goat
    Sample Types: Whole Cell
    Applications: IHC
  52. Creatine modulates cellular energy metabolism and protects against cancer cachexia-associated muscle wasting
    Authors: L Wei, R Wang, K Lin, X Jin, L Li, J Wazir, W Pu, P Lian, R Lu, S Song, Q Zhao, J Li, H Wang
    Frontiers in Pharmacology, 2022-12-07;13(0):1086662.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  53. MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2
    Authors: J Shen, J Wang, H Zhen, Y Liu, L Li, Y Luo, J Hu, X Liu, S Li, Z Hao, M Li, Z Zhao
    International Journal of Molecular Sciences, 2022-11-05;23(21):.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: IHC
  54. Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration
    Authors: W Chen, W Chen, P Liu, S Qian, S Tao, M Huang, W Xu, C Li, X Chen, H Lin, Z Qin, J Lu, S Xie
    Cells, 2022-11-04;11(21):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  55. Celecoxib impairs primary human myoblast proliferation and differentiation independent of cyclooxygenase 2 inhibition
    Authors: RW Matheny, AL Kolb, AV Geddis, BM Roberts
    Physiological Reports, 2022-11-01;10(21):e15481.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. Protective Effects of the Chalcone-Based Derivative AN07 on Inflammation-Associated Myotube Atrophy Induced by Lipopolysaccharide
    Authors: WY Fang, CL Lin, WH Chang, CH Chang, YC Huang, YH Tsai, FR Chang, YC Lo
    International Journal of Molecular Sciences, 2022-10-26;23(21):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  57. 2-Deoxy-D-glucose Alleviates Cancer Cachexia-Induced Muscle Wasting by Enhancing Ketone Metabolism and Inhibiting the Cori Cycle
    Authors: L Wei, R Wang, J Wazir, K Lin, S Song, L Li, W Pu, C Zhao, Y Wang, Z Su, H Wang
    Cells, 2022-09-25;11(19):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  58. Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development
    Authors: M Ling, X Lai, L Quan, F Li, L Lang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang
    International Journal of Molecular Sciences, 2022-07-07;23(14):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  59. COPS3 AS lncRNA enhances myogenic differentiation and maintains fast-type myotube phenotype
    Authors: ZZ He, T Zhao, N Qimuge, T Tian, W Yan, X Yi, J Jin, R Cai, T Yu, G Yang, W Pang
    Cellular Signalling, 2022-04-28;95(0):110341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  60. Knockdown of CDR1as Decreases Differentiation of Goat Skeletal Muscle Satellite Cells via Upregulating miR-27a-3p to Inhibit ANGPT1
    Authors: B Kyei, E Odame, L Li, L Yang, S Zhan, J Li, Y Chen, D Dai, J Cao, J Guo, T Zhong, L Wang, H Zhang
    Genes, 2022-04-09;13(4):.
    Species: Goat
    Sample Types: Transfected Whole Cells
    Applications: ICC
  61. Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells
    Authors: I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur
    Science Advances, 2022-04-06;8(14):eabj4928.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  62. Tceal5 and Tceal7 Function in C2C12 Myogenic Differentiation via Exosomes in Fetal Bovine Serum
    Authors: A Sawada, T Yamamoto, T Sato
    International Journal of Molecular Sciences, 2022-02-12;23(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  63. Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration
    Authors: X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang
    Disease Models & Mechanisms, 2021-12-24;14(12):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  64. beta-Hydroxy-beta-Methylbutyrate Supplementation Promotes Antitumor Immunity in an Obesity Responsive Mouse Model of Pancreatic Ductal Adenocarcinoma
    Authors: MF Coleman, KA Liu, AJ Pfeil, SK Etigunta, X Tang, S Fabela, LM Lashinger, Z Cui, SD Hursting
    Cancers, 2021-12-18;13(24):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway
    Authors: M Ling, L Quan, X Lai, L Lang, F Li, X Yang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang
    International Journal of Molecular Sciences, 2021-12-12;22(24):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  66. Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia
    Authors: K Xie, H Xiong, W Xiao, Z Xiong, W Hu, J Ye, N Xu, J Shi, C Yuan, Z Chen, D Miao, X Zhang, H Yang
    Cancer Cell International, 2021-11-27;21(1):627.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  67. Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test
    Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
    Toxicology and Applied Pharmacology, 2021-10-29;433(0):115781.
    Species: Mouse
    Sample Types: Embryonic Bodies
    Applications: ICC
  68. M2 Macrophage-derived exosomal miR-501 contributes to pubococcygeal muscle regeneration
    Authors: M Zhou, B Li, C Liu, M Hu, J Tang, J Min, J Cheng, L Hong
    International immunopharmacology, 2021-10-08;101(0):108223.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  69. Simple derivation of skeletal muscle from human pluripotent stem cells using temperature-sensitive Sendai virus vector
    Authors: GW Tan, T Kondo, K Imamura, M Suga, T Enami, A Nagahashi, K Tsukita, I Inoue, J Kawaguchi, T Shu, H Inoue
    Journal of Cellular and Molecular Medicine, 2021-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  70. The ubiquitin ligase Ozz decreases the replacement rate of embryonic myosin in myofibrils
    Authors: E Ichimura, K Ojima, S Muroya, T Suzuki, K Kobayashi, T Nishimura
    Physiological Reports, 2021-09-01;9(17):e15003.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  71. Wharton's Jelly-Derived Mesenchymal Stem Cells Reduce Fibrosis in a Mouse Model of Duchenne Muscular Dystrophy by Upregulating microRNA 499
    Authors: SE Park, JB Jeong, SJ Oh, SJ Kim, H Kim, A Choi, SJ Choi, SY Oh, GH Ryu, J Lee, HB Jeon, JW Chang
    Biomedicines, 2021-08-26;9(9):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  72. Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
    Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
    Toxicology reports, 2021-07-31;8(0):1513-1520.
    Species: Mouse
    Sample Types: Embryoid Bodies
    Applications: ICC
  73. Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway
    Authors: Y She, C Li, T Jiang, S Lei, S Zhou, H Shi, R Chen
    Frontiers in Physiology, 2021-07-08;12(0):659272.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  74. An integrative transcriptome study reveals Ddit4/Redd1 as a key regulator of cancer cachexia in rodent models
    Authors: M Niu, L Li, Z Su, L Wei, W Pu, C Zhao, Y Ding, J Wazir, W Cao, S Song, Q Gao, H Wang
    Cell Death & Disease, 2021-06-26;12(7):652.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  75. Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control
    Authors: B Lee, S Lee, Y Lee, Y Park, J Shim
    International Journal of Molecular Sciences, 2021-06-22;22(13):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  76. Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle
    Authors: K Yoshioka, H Nagahisa, F Miura, H Araki, Y Kamei, Y Kitajima, D Seko, J Nogami, Y Tsuchiya, N Okazaki, A Yonekura, S Ohba, Y Sumita, K Chiba, K Ito, I Asahina, Y Ogawa, T Ito, Y Ohkawa, Y Ono
    Science Advances, 2021-06-09;7(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  77. The Effects of Muscle Cell Aging on Myogenesis
    Authors: A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier
    International Journal of Molecular Sciences, 2021-04-02;22(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  78. Neural stemness contributes to cell tumorigenicity
    Authors: L Xu, M Zhang, L Shi, X Yang, L Chen, N Cao, A Lei, Y Cao
    Cell & bioscience, 2021-01-19;11(1):21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  79. FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling
    Authors: A Knyazeva, A Khudiakov, R Vaz, A Muravyev, K Sukhareva, T Sejersen, A Kostareva
    Genes (Basel), 2020-11-13;11(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  80. Daily Oral Administration of Protease-Treated Royal Jelly Protects Against Denervation-Induced Skeletal Muscle Atrophy
    Authors: T Shirakawa, A Miyawaki, T Matsubara, N Okumura, H Okamoto, N Nakai, T Rojasawast, K Morikawa, A Inoue, A Goto, A Washio, T Tsujisawa, T Kawamoto, S Kokabu
    Nutrients, 2020-10-11;12(10):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  81. The calmodulin redox sensor controls myogenesis
    Authors: AW Steil, JW Kailing, CJ Armstrong, DG Walgenbach, JC Klein
    PLoS ONE, 2020-09-17;15(9):e0239047.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  82. LMNA Mutations G232E and R482L Cause Dysregulation of Skeletal Muscle Differentiation, Bioenergetics, and Metabolic Gene Expression Profile
    Authors: EV Ignatieva, OA Ivanova, MY Komarova, NV Khromova, DE Polev, AA Kostareva, A Sergushich, RI Dmitrieva
    Genes (Basel), 2020-09-07;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  83. In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix
    Authors: J Lee, A Sutani, R Kaneko, J Takeuchi, T Sasano, T Kohda, K Ihara, K Takahashi, M Yamazoe, T Morio, T Furukawa, F Ishino
    Nat Commun, 2020-09-03;11(1):4283.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  84. Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells
    Authors: D Baci, M Chirivì, V Pace, F Maiullari, M Milan, A Rampin, P Somma, D Presutti, S Garavelli, A Bruno, S Cannata, C Lanzuolo, C Gargioli, R Rizzi, C Bearzi
    Cells, 2020-06-23;9(6):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  85. Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration
    Authors: C Imashiro, M Hirano, T Morikura, Y Fukuma, K Ohnuma, Y Kurashina, S Miyata, K Takemura
    Sci Rep, 2020-06-11;10(1):9468.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  86. Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors
    Authors: Y Nakatake, SBH Ko, AA Sharov, S Wakabayash, M Murakami, M Sakota, N Chikazawa, C Ookura, S Sato, N Ito, M Ishikawa-H, SS Mak, LM Jakt, T Ueno, K Hiratsuka, M Matsushita, SK Goparaju, T Akiyama, KI Ishiguro, M Oda, N Gouda, A Umezawa, H Akutsu, K Nishimura, R Matoba, O Ohara, MSH Ko
    Cell Rep, 2020-05-19;31(7):107655.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  87. Urolithin A exerts antiobesity effects through enhancing adipose tissue thermogenesis in mice
    Authors: B Xia, XC Shi, BC Xie, MQ Zhu, Y Chen, XY Chu, GH Cai, M Liu, SZ Yang, GA Mitchell, WJ Pang, JW Wu
    PLoS Biol., 2020-03-27;18(3):e3000688.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  88. Corylifol A from Psoralea corylifolia L. Enhances Myogenesis and Alleviates Muscle Atrophy
    Authors: Y Han, H Lee, H Li, JH Ryu
    Int J Mol Sci, 2020-02-25;21(5):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  89. CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Authors: H Morisaka, K Yoshimi, Y Okuzaki, P Gee, Y Kunihiro, E Sonpho, H Xu, N Sasakawa, Y Naito, S Nakada, T Yamamoto, S Sano, A Hotta, J Takeda, T Mashimo
    Nat Commun, 2019-12-06;10(1):5302.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  90. The SMYD3 methyltransferase promotes myogenesis by activating the myogenin regulatory network
    Authors: R Codato, M Perichon, A Divol, E Fung, A Sotiropoul, A Bigot, JB Weitzman, S Medjkane
    Sci Rep, 2019-11-21;9(1):17298.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  91. Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens
    Authors: Y Nihashi, K Umezawa, S Shinji, Y Hamaguchi, H Kobayashi, T Kono, T Ono, H Kagami, T Takaya
    Sci Rep, 2019-11-11;9(1):16527.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: ICC
  92. FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis
    Authors: G Picchiarel, M Demestre, A Zuko, M Been, J Higelin, S Dieterlé, MA Goy, M Mallik, C Sellier, J Scekic-Zah, L Zhang, A Rosenbohm, C Sijlmans, A Aly, S Mersmann, I Sanjuan-Ru, A Hübers, N Messaddeq, M Wagner, N van Bakel, AL Boutillier, A Ludolph, C Lagier-Tou, TM Boeckers, L Dupuis, E Storkebaum
    Nat. Neurosci., 2019-10-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Expression patterns of regulatory lncRNAs and miRNAs in muscular atrophy models induced by starvation in vitro and in vivo
    Authors: S Lei, Y She, J Zeng, R Chen, S Zhou, H Shi
    Mol Med Rep, 2019-09-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ICC, Western Blot
  94. Skeletal Muscle Atrophy Was Alleviated by Salidroside Through Suppressing Oxidative Stress and Inflammation During Denervation
    Authors: Z Huang, Q Fang, W Ma, Q Zhang, J Qiu, X Gu, H Yang, H Sun
    Front Pharmacol, 2019-09-10;10(0):997.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ICC, Western Blot
  95. The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells
    Authors: SY Lee, J Yang, JH Park, HK Shin, WJ Kim, SY Kim, EJ Lee, I Hwang, CS Lee, J Lee, HS Kim
    Mol. Ther., 2019-09-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  96. Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise
    Authors: Y Zhang, Y Liu, X Bi, C Hu, F Ding, W Ding
    Mediators Inflamm., 2019-08-18;2019(0):2789014.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  97. Increase in HDAC9 suppresses myoblast differentiation via epigenetic regulation of autophagy in hypoxia
    Authors: Z Zhang, L Zhang, Y Zhou, L Li, J Zhao, W Qin, Z Jin, W Liu
    Cell Death Dis, 2019-07-18;10(8):552.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  98. RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs
    Authors: Y Komatsu, D Takeuchi, T Tokunaga, H Sakurai, A Makino, T Honda, Y Ikeda, K Tomonaga
    Mol Ther Methods Clin Dev, 2019-05-28;14(0):47-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  99. Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs
    Authors: D Abbadi, M Yang, DM Chenette, JJ Andrews, RJ Schneider
    Proc. Natl. Acad. Sci. U.S.A., 2019-05-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  100. IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway
    Authors: D Liu, X Qiao, Z Ge, Y Shang, Y Li, W Wang, M Chen, S Si, SZ Chen
    Skelet Muscle, 2019-03-28;9(1):8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  101. MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
    Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
    Cell Stem Cell, 2019-03-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  102. Epigenetic regulation of the PTEN-AKT-RAC1 axis by G9a is critical for tumor growth in alveolar rhabdomyosarcoma
    Authors: AV Bhat, M Palanicham, VK Rao, L Pignata, HJ Lim, S Suriyamurt, KTE Chang, VK Lee, E Guccione, R Taneja
    Cancer Res., 2019-03-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  103. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects
    Authors: J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi
    Cell Rep, 2019-02-26;26(9):2494-2508.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  104. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy
    Authors: MC Estañ, E Fernández-, MS Zaki, MI Esteban, S Donkervoor, C Hawkins, JA Caparros-M, D Saade, Y Hu, V Bolduc, KR Chao, J Nevado, A Lamuedra, R Largo, G Herrero-Be, J Regadera, C Hernandez-, EF Tizzano, V Martinez-G, JJ Carvajal, R Zong, DL Nelson, GA Otaify, S Temtamy, M Aglan, M Issa, CG Bönnemann, P Lapunzina, G Yoon, VL Ruiz-Perez
    Nat Commun, 2019-02-15;10(1):797.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  105. p300 mediates muscle wasting in Lewis lung carcinoma
    Authors: YP Li, TK Sin, JZ Zhu, G Zhang
    Cancer Res., 2019-01-31;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  106. Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
    Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
    Stem Cells Int, 2019-01-03;2019(0):5690345.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  107. Pannexin 1 inhibits rhabdomyosarcoma progression through a mechanism independent of its canonical channel function
    Authors: X Xiang, S Langlois, ME St-Pierre, JF Barré, D Grynspan, B Purgina, KN Cowan
    Oncogenesis, 2018-11-21;7(11):89.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  108. Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons
    Authors: T Osaki, SGM Uzel, RD Kamm
    Sci Adv, 2018-10-10;4(10):eaat5847.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  109. The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice
    Authors: T Sasaki, A Kuboyama, M Mita, S Murata, M Shimizu, J Inoue, K Mori, R Sato
    J. Biol. Chem., 2018-05-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  110. Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells
    Authors: R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin
    Stem Cells Dev., 2018-05-09;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P
  111. Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors
    Authors: F Sakai-Take, A Narita, S Masuda, T Wakamatsu, N Watanabe, T Nishiyama, K Nogami, M Blanc, S Takeda, Y Miyagoe-Su
    Sci Rep, 2018-04-26;8(1):6555.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  112. HSP90 modulates the myosin replacement rate in myofibrils
    Authors: K Ojima, E Ichimura, T Suzuki, M Oe, S Muroya, T Nishimura
    Am. J. Physiol., Cell Physiol., 2018-03-21;0(0):.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Western Blot
  113. OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13
    Authors: B Kalbe, M Osterloh, VM Schulz, J Altmüller, C Becker, S Osterloh, H Hatt
    Arch. Biochem. Biophys., 2018-03-17;645(0):72-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  114. Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia
    Authors: R Chen, J Xu, Y She, T Jiang, S Zhou, H Shi, C Li
    Int. J. Mol. Med., 2018-02-06;41(5):2565-2572.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  115. Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients
    Authors: M Wattin, L Gaweda, P Muller, M Baritaud, C Scholtes, C Lozano, K Gieseler, C Kretz-Remy
    Int J Mol Sci, 2018-01-07;19(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  116. The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts
    Authors: H Sakane, K Akasaki
    Biol. Pharm. Bull., 2018-01-01;41(8):1186-1193.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  117. Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration
    Authors: M Koide, Y Hagiwara, M Tsuchiya, M Kanzaki, H Hatakeyama, Y Tanaka, T Minowa, T Takemura, A Ando, T Sekiguchi, Y Yabe, E Itoi
    Tohoku J. Exp. Med., 2018-01-01;244(1):15-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  118. Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy
    Authors: H Lee, SJ Lee, GU Bae, NI Baek, JH Ryu
    Int J Mol Sci, 2017-12-18;18(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  119. Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
    Authors: G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li
    Nat Commun, 2017-09-19;8(1):589.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  120. NF?B signaling in alveolar rhabdomyosarcoma
    Authors: MM Cleary, A Mansoor, T Settelmeye, Y Ijiri, KJ Ladner, MN Svalina, BP Rubin, DC Guttridge, C Keller
    Dis Model Mech, 2017-09-01;10(9):1109-1115.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  121. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor
    Authors: S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi
    J. Biol. Chem., 2017-06-12;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  122. Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation
    Authors: DS Han, WS Yang, TW Kao
    Int J Med Sci, 2017-04-08;14(5):434-443.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  123. R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways
    Authors: F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand
    Cell Rep, 2017-03-07;18(10):2320-2330.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  124. Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability
    Authors: J Ueki, M Nakamori, M Nakamura, M Nishikawa, Y Yoshida, A Tanaka, A Morizane, M Kamon, T Araki, MP Takahashi, A Watanabe, N Inagaki, H Sakurai
    Sci Rep, 2017-02-13;7(0):42522.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  125. CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  126. Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation
    Authors: Hiroyuki Tsutsui
    Am. J. Physiol., Cell Physiol., 2016-11-02;0(0):ajpcell.00090.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  127. Activin A induces skeletal muscle catabolism via p38? mitogen-activated protein kinase
    Authors: Yi-Ping Li
    J Cachexia Sarcopenia Muscle, 2016-09-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  128. Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1
    Sci Rep, 2016-08-23;6(0):32084.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  129. Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation
    Sci Rep, 2016-06-28;6(0):28829.
    Species: Human, Rat
    Sample Types: Protein
    Applications: Western Blot
  130. Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma
    Authors: A Kim, M Im, MJ Gu, JY Ma
    Sci Rep, 2016-04-11;6(0):24214.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  131. Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
    Authors: Vleeshouwer-Neumann T, Phelps M, Bammler T, Macdonald J, Jenkins I, Chen E
    PLoS ONE, 2015-12-04;10(12):e0144320.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  132. Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
    Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
    Diabetes, 2015-12-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Simple Western
  133. Molecular ties between the cell cycle and differentiation in embryonic stem cells.
    Authors: Li, Victor C, Kirschner, Marc W
    Proc Natl Acad Sci U S A, 2014-06-16;111(26):9503-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  134. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014-04-01;184(4):1152-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  135. Myoblasts Derived From Normal hESCs and Dystrophic hiPSCs Efficiently Fuse With Existing Muscle Fibers Following Transplantation.
    Authors: Goudenege S, Lebel C, Huot N, Dufour C, Fujii I, Gekas J, Rousseau J, Tremblay J
    Mol Ther, 2012-09-18;20(11):2153-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  136. Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
    Authors: Joe MK, Kee C, Tomarev SI
    J. Biol. Chem., 2012-02-25;287(16):13216-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  137. Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
    Authors: Ono Y, Masuda S, Nam HS
    J. Cell. Sci., 2012-02-20;125(0):1309-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  138. Magnolol Attenuates Cisplatin-Induced Muscle Wasting by M2c Macrophage Activation
    Authors: Chanju Lee, Hyunju Jeong, Hyunji Lee, Minwoo Hong, Seon-young Park, Hyunsu Bae
    Frontiers in Immunology
  139. Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis
    Authors: Cai R, Zhang Q, Wang Y et al.
    The Journal of biological chemistry
  140. Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes
    Authors: Cliff I. Stains, Nathan C. Tedford, Traci C. Walkup, Elvedin Luković, Brenda N. Goguen, Linda G. Griffith et al.
    Chemistry & Biology
  141. CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex
    Authors: Huang R, Yamamoto T, Nakata E et al.
    Advanced science (Weinheim, Baden-Wurttemberg, Germany)
  142. Human iPS Cells Derived Skeletal Muscle Progenitor Cells Promote Myoangiogenesis and Restore Dystrophin in Duchenne Muscular Dystrophic Mice
    Authors: Khan M, Ashraf M
    Stem Cell Res Ther
  143. Active tissue adhesive activates mechanosensors and prevents muscle atrophy
    Authors: Sungmin Nam, Bo Ri Seo, Alexander J. Najibi, Stephanie L. McNamara, David J. Mooney
    Nature Materials
  144. Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-kappa B and regulating protein synthesis/degradation
    Authors: Xiangjiao Yi, Jianguo Tao, Yu Qian, Feng Feng, Xueqin Hu, Taotao Xu et al.
    Frontiers in Pharmacology
  145. Cancer-induced muscle wasting requires p38beta MAPK activation of p300.
    Authors: Sin, T K, Zhang, G Et al.
    Cancer Res
  146. Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells
    Authors: Xiangping Yao, Taiyong Yu, Chen Zhao, Youlei Li, Ying Peng, Fengxue Xi et al.
    International Journal of Molecular Medicine
  147. Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia
    Authors: Mengistu Lemecha, Jaya Prakash Chalise, Yuki Takamuku, Guoxiang Zhang, Takahiro Yamakawa, Garrett Larson et al.
    Molecular Metabolism
  148. SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1 alpha pathway
    Authors: Xiaofan Yang, Pingping Xue, Zhenyu Liu, Wenqing Li, Chuyan Li, Zhenbing Chen
    Cellular & Molecular Biology Letters
  149. Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquine
    Authors: Ulka Sachdev, Ricardo Ferrari, Xiangdong Cui, Abish Pius, Amrita Sahu, Michael Reynolds et al.
    Molecular Medicine
  150. Pharmacological Inhibition of HMGB1 Prevents Muscle Wasting
    Authors: Lu Li, Huiquan Liu, Weili Tao, Su Wen, Xiaofen Fu, Shiying Yu
    Frontiers in Pharmacology
  151. Z-ajoene from Crushed Garlic Alleviates Cancer-Induced Skeletal Muscle Atrophy
    Authors: Hyejin Lee, Ji-Won Heo, A-Reum Kim, Minson Kweon, Sorim Nam, Jong-Seok Lim et al.
    Nutrients
  152. Dimethyl-2-oxoglutarate improves redox balance and mitochondrial function in muscle pericytes of individuals with diabetes mellitus
    Authors: Ashton Faulkner, Anita Tamiato, William Cathery, Andrea Rampin, Carlo Maria Caravaggi, Eva Jover et al.
    Diabetologia
  153. Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells
    Authors: Yuko Kokubu, Tomoko Nagino, Katsunori Sasa, Tatsuo Oikawa, Katsuya Miyake, Akiko Kume et al.
    Stem Cells Translational Medicine
  154. Generation of Functional Myocytes from Equine Induced Pluripotent Stem Cells
    Authors: Karin R. Amilon, Yennifer Cortes-Araya, Benjamin Moore, Seungmee Lee, Simon Lillico, Amandine Breton et al.
    Cellular Reprogramming
  155. Feeder-supported in vitro exercise model using human satellite cells from patients with sporadic inclusion body myositis
    Authors: Y Li, W Chen, K Ogawa, M Koide, T Takahashi, Y Hagiwara, E Itoi, T Aizawa, M Tsuchiya, R Izumi, N Suzuki, M Aoki, M Kanzaki
    Scientific Reports, 2022-01-20;12(1):1082.
  156. Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells’ Proliferation through Regulating the Cell Cycle
    Authors: Yiren Jiao, Bo Huang, Yu Chen, Guangliang Hong, Jian Xu, Chingyuan Hu et al.
    International Journal of Molecular Sciences
  157. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice
    Authors: Zhao W, Chen L, Tan W et al.
    Foods
  158. Skeletal muscle releases extracellular vesicles with distinct protein and miRNA signatures that function in the muscle microenvironment
    Authors: Watanabe S, Sudo Y, Makino T, Kimura S
    PNAS

FAQs

  1. Does Myosin Heavy Chain Antibody, Catalog # MAB4470, detect Myosin Heavy Chain in all mammalian species?

    • Myosin Heavy Chain is a highly conserved protein sequence. Although we have not tested every mammalian species, we expect it will bind to Myosin Heavy Chain in all mammalian species.

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Myosin Heavy Chain Antibody
By Anonymous on 03/29/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Muscle tissue Species: Human
Myosin Heavy Chain Antibody
By Anonymous on 08/02/2021
Application: IHC Sample Tested: Muscle tissue Species: Mouse

Immunofluorescence, mouse muscle tissue. On the photo: Myosin Heavy Chain Antibody in red, GAP43 in green.

Myosin Heavy Chain Antibody
By Anonymous on 12/04/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Myotubes Species: horse
Immunocytochemistry/Immunofluorescence Myosin Heavy Chain Antibody MAB4470
Myosin Heavy Chain Antibody
By Anonymous on 10/29/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skeletal muscle Species: Mouse
Immunocytochemistry/Immunofluorescence Myosin Heavy Chain Antibody MAB4470
Myosin Heavy Chain Antibody
By Anonymous on 10/23/2020
Application: IHC Sample Tested: Heart tissue Species: Mouse

Heart tissue was fixed with paraformaldehyde and embedded with paraffin. The section was pre-treated with heart induced antigen retrieval and blocked with 10% goat serum and 3% BSA. Then the section was incubated with MAB4470 with a concentration of 10 ug/mL at 4C overnight and incubated with anti-mouse Alexa Fluo 488 secondary antiobody for 1 hour at room temperature. Nuclei were stained with DAPI.

Immunohistochemistry Myosin Heavy Chain Antibody MAB4470
Myosin Heavy Chain Antibody
By Anonymous on 06/06/2019
Application: WB Sample Tested: C2C12 mouse myoblast cell line,Differentiated C2C12 myoblast cells Species: Mouse
Western Blot Myosin Heavy Chain Antibody MAB4470
Myosin Heavy Chain Antibody
By Michelle Chen on 05/10/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skeletal muscle tissue Species: Human