Mouse ZIP4H/Tex11 Antibody

Catalog # Availability Size / Price Qty
AF5627
AF5627-SP
Detection of Mouse ZIP4H/Tex11 by Western Blot.
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Mouse ZIP4H/Tex11 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ZIP4H/Tex11 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse ZIP4H/Tex11
Met768-Leu947
Accession # Q14AT2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ZIP4H/Tex11 antibody by Western Blot. View Larger

Detection of Mouse ZIP4H/Tex11 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected, transfected with human ZIP4H, or transfected with mouse ZIP4H. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse ZIP4H/Tex11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5627) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZIP4H/Tex11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZIP4H/Tex11

ZIP4 homolog (ZIP4H), also known as testis expressed sequence 11 (Tex11) is a 947 amino acid (aa) X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa 691‑707) and truncation (aa 708‑947) at the C-terminus. Within aa 768‑947, mouse ZIP4H shares 44% and 73% aa sequence identity with human and rat ZIP4H, respectively.

Long Name
Zinc Transporter Protein 4 Homolog
Entrez Gene IDs
56159 (Human); 83558 (Mouse); 501588 (Rat)
Alternate Names
testis expressed 11; testis expressed sequence 11; testis-expressed sequence 11 protein; Tex11; TGC1; TSGA3; ZIP4H

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