Mouse VEGF Biotinylated Antibody Summary
Ala27-Arg190
Accession # AAA40547
Applications
Mouse VEGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF
Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.
Product Datasheets
Citations for Mouse VEGF Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration
Authors: A Yaguchi, M Oshikawa, G Watanabe, H Hiramatsu, N Uchida, C Hara, N Kaneko, K Sawamoto, T Muraoka, I Ajioka
Nature Communications, 2021-11-19;12(1):6623.
Species: Mouse
Sample Types: Protein
Applications: ELISA Detection -
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
Exp Cell Res, 2014-02-22;323(1):7-27.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
Genes Dev., 2006-09-15;20(18):2527-38.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Application of plasmid DNA encoding IL-18 diminishes development of herpetic stromal keratitis by antiangiogenic effects.
Authors: Kim B, Lee S, Suvas S, Rouse BT
J. Immunol., 2005-07-01;175(1):509-16.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Flow Cytometry -
Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors
Authors: JR Lee, I Appelmann, C Miething, TO Shultz, D Ruderman, D Kim, P Mallick, SW Lowe, SX Wang
Theranostics, 2018-02-03;8(5):1389-1398.
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The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells
Authors: Leslee Sprague, Maria Muccioli, Michelle Pate, Evan Meles, John McGinty, Harika Nandigam et al.
BMC Immunology
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Experimental and theoretical investigation of the precise transduction mechanism in giant magnetoresistive biosensors
Authors: Jung-Rok Lee, Noriyuki Sato, Daniel J. B. Bechstein, Sebastian J. Osterfeld, Junyi Wang, Adi Wijaya Gani et al.
Scientific Reports
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Adhesion to substrates induces dendritic cell endothelization and decreases immunological response
Authors: Jacob Osterbur, Leslee Sprague, Maria Muccioli, Michelle Pate, Kristen Mansfield, John McGinty et al.
Immunobiology
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0.4ug/ml dilution in 1% BSA PBST, 1 hour incubation
